在注射神经生长因子（NGF）进行前交叉韧带重建后，通过组织病理学和电生理评估测量的本体感觉变化。

Proprioceptive changes measured by histopathological and electrophysiological evaluations after NGF injection of anterior cruciate ligament reconstruction.

作者信息

Zhen Dong, Qiu Bing, Wang Weiguo, Yang Zhirong, Wang Jingwei, Gan Yang, Qiu Bing

机构信息

Department of Sports Medicine, Orthopedic Hospital of Guizhou Province, Guiyang 550002, China.

Department of Pathology, Orthopedic Hospital of Guizhou Province, Guiyang 550002, China.

出版信息

Ann Palliat Med. 2020 Jan;9(1):37-44. doi: 10.21037/apm.2020.01.06.

DOI:10.21037/apm.2020.01.06

PMID:32005061

Abstract

BACKGROUND

Proprioceptive recovery has received an increased amount of attention after undergoing anterior cruciate ligament (ACL) reconstruction. However, ACL reconstruction without rehabilitation training could not significantly improve the reduction and function of the proprioceptor. This study aimed to explore whether nerve growth factor (NGF) could cause proprioceptive changes after ACL reconstruction through histopathological and electrophysiological evaluations.

METHODS

A total 28 mature New Zealand white rabbits were used in the study, 24 to develop the model of ACL injury and ACL reconstruction. These included the experimental group (n=12; injected with NGF 20 µg/week at the second month after surgery) and the experimental control group (n=12), and 4 for blank control group. In the 4th, 8th, and 12th months, the changes in ACL nerves were measured by somatosensory evoked potentials (SEPs) and electromyogram (EMG). Furthermore, gold chloride staining was performed to detect the changes in morphology and quantity of the proprioceptors in ACL.

RESULTS

Electrophysiological tests showed that the incubation of SEPs and EMG in both the experimental group and the experimental control group were prolonged, and the amplitude decreased when compared with the blank control group (all, P<0.05). Also, in comparison to the experimental control group, the experimental group injected with NGF had a shorter incubation and higher amplitude (all, P<0.05). Furthermore, histopathology analysis revealed that the number of proprioceptors in the experimental group injected with NGF was significantly increased, and the atypical structure was reduced (all, P<0.05).

CONCLUSIONS

The results showed the injection of NGF (injected with 20 µg/week in the second month after surgery) could improve joint function rehabilitation by promoting function and quantity of proprioception after ACL reconstruction.

摘要

背景

前交叉韧带（ACL）重建术后本体感觉的恢复受到了越来越多的关注。然而，未经康复训练的ACL重建并不能显著改善本体感受器的恢复和功能。本研究旨在通过组织病理学和电生理评估，探讨神经生长因子（NGF）是否能在ACL重建后引起本体感觉的变化。

方法

本研究共使用28只成年新西兰白兔，其中24只用于建立ACL损伤和ACL重建模型。这些兔子分为实验组（n=12；术后第二个月每周注射20μg NGF）和实验对照组（n=12），4只为空白对照组。在第4、8和12个月，通过体感诱发电位（SEPs）和肌电图（EMG）测量ACL神经的变化。此外，进行氯化金染色以检测ACL中本体感受器的形态和数量变化。