Laboratory of Farm Animal Production Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
Laboratory of Animal Hygiene, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
Microbiol Immunol. 2020 May;64(5):387-391. doi: 10.1111/1348-0421.12779. Epub 2020 Feb 24.
Because broad genetic diversity has recently been detected in Torque teno sus viruses (TTSuV1 and TTSuVk2), the viral genome detection method needs to be improved to understand the prevalence of these viruses. Here, we established single PCR-based detection methods for the TTSuV1 and TTSuVk2a genomes with newly designed primer pairs and applied them to investigate the prevalence of TTSuV1 and TTSuVk2a in Japanese pig populations. The results revealed that 98.2% and 81.7% of the pig farms tested positive for the TTSuV1 and TTSuVk2a genomes, respectively, indicating that both TTSuV1 and TTSuVk2a are widespread in Japan.
由于最近在扭结瘤病毒（TTSuV1 和 TTSuVk2）中检测到广泛的遗传多样性，因此需要改进病毒基因组检测方法，以了解这些病毒的流行情况。在这里，我们使用新设计的引物对建立了用于 TTSuV1 和 TTSuVk2a 基因组的基于单个 PCR 的检测方法，并将其应用于研究日本猪群中 TTSuV1 和 TTSuVk2a 的流行情况。结果表明，分别有 98.2%和 81.7%的猪场检测到 TTSuV1 和 TTSuVk2a 基因组呈阳性，表明 TTSuV1 和 TTSuVk2a 在日本均广泛存在。
