Department of Neurology, Wuhan University, Renmin Hospital, Wuhan, Hubei Province, P.R. China.
Int J Neurosci. 2020 Nov;130(11):1109-1117. doi: 10.1080/00207454.2020.1725511. Epub 2020 Feb 10.
Guillain-Barré syndrome (GBS), an autoimmune disease and an acute inflammation disorder, is currently the most frequent cause of acute flaccid paralysis worldwide. EAN, an animal model of GBS, is a CD4+ T cell-mediated autoimmune disease of the PNS. Wnt/β-catenin signals are critically important to several fundamental aspects of peripheral nerve development and play a crucial role in Schwann cell proliferation. Here, we investigate the role of Wnt/β-catenin signalling cascades in EAN rats. 28 male Lewis rats weighing 170 ± 10 g were randomly divided into control group ( = 7) and EAN groups (Early group; Peak group and Recovery group. = 7 per group). EAN rats were immunized with P2 peptide; weighed daily, and the neurologic signs of EAN were evaluated every day. The sciatic nerve was taken on the days 10, 17, and 30 p.i. for H&E staining, transmission electron microscopy and immunohistochemical staining; blood samples were collected weekly from caudal vein to detect IFN-γ, IL-4, TGF-β1; and the sciatic nerve was taken to examinate the dynamics expression of Wnt/β-catenin pathway molecules. In our study, we chose tail-root injection to better model GBS. Moreover, we observed that IFN-γ levels paralleled clinical EAN, and the levels of TGF-β1 and IL-4 gradually increased and peaked in the recovery phase. In addition, we have shown that canonical Wnt signalling is upregulated and reached a peak in the late recovery phase. Our findings suggest that Wnt/β-catenin signalling is associated with the promotion of remyelination in EAN rats.
格林-巴利综合征（GBS）是一种自身免疫性疾病和急性炎症性疾病，是目前全球最常见的急性弛缓性瘫痪的原因。EAN，GBS 的动物模型，是一种 CD4+T 细胞介导的周围神经系统自身免疫性疾病。Wnt/β-连环蛋白信号对于周围神经发育的几个基本方面至关重要，并在 Schwann 细胞增殖中发挥关键作用。在这里，我们研究了 Wnt/β-连环蛋白信号通路在 EAN 大鼠中的作用。28 只体重为 170 ± 10 g 的雄性 Lewis 大鼠被随机分为对照组（n = 7）和 EAN 组（早期组；高峰组和恢复期组，每组 n = 7）。EAN 大鼠用 P2 肽免疫；每天称重，并每天评估 EAN 的神经症状。在感染后第 10、17 和 30 天取坐骨神经进行 H&E 染色、透射电镜和免疫组织化学染色；每周从尾静脉采集血液样本以检测 IFN-γ、IL-4、TGF-β1；取坐骨神经检测 Wnt/β-连环蛋白通路分子的动态表达。在我们的研究中，我们选择尾根注射来更好地模拟 GBS。此外，我们观察到 IFN-γ 水平与临床 EAN 平行，而 TGF-β1 和 IL-4 的水平在恢复期逐渐升高并达到峰值。此外，我们已经表明，经典 Wnt 信号通路被上调，并在后期恢复阶段达到峰值。我们的研究结果表明，Wnt/β-连环蛋白信号通路与 EAN 大鼠的髓鞘再生促进有关。
