利用拉曼光谱和表面增强拉曼光谱鉴别小鼠淋巴瘤细胞的放射敏感性和放射抗性
Discrimination of radiosensitive and radioresistant murine lymphoma cells by Raman spectroscopy and SERS.
作者信息
Aguilar-Hernández Iris, Cárdenas-Chavez Diana L, López-Luke Tzarara, García-García Alejandra, Herrera-Domínguez Marcela, Pisano Eduardo, Ornelas-Soto Nancy
机构信息
Laboratorio de Nanotecnología Ambiental, Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey, N.L. 64849, Mexico.
Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Atlixcáyotl 5718, Puebla, Pue., México, 72453, Mexico.
出版信息
Biomed Opt Express. 2019 Dec 23;11(1):388-405. doi: 10.1364/BOE.11.000388. eCollection 2020 Jan 1.
Intrinsic radiosensitivity is a biological parameter known to influence the response to radiation therapy in cancer treatment. In this study, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) were successfully used in conjunction with principal component analysis (PCA) to discriminate between radioresistant (LY-R) and radiosensitive (LY-S) murine lymphoma sublines (L5178Y). PCA results for normal Raman analysis showed a differentiation between the radioresistant and radiosensitive cell lines based on their specific spectral fingerprint. In the case of SERS with gold nanoparticles (AuNPs), greater spectral enhancements were observed in the radioresistant subline in comparison to its radiosensitive counterpart, suggesting that each subline displays different interaction with AuNPs. Our results indicate that spectroscopic and chemometric techniques could be used as complementary tools for the prediction of intrinsic radiosensitivity of lymphoma samples.
内在放射敏感性是一种已知会影响癌症治疗中放射治疗反应的生物学参数。在本研究中，拉曼光谱和表面增强拉曼光谱（SERS）与主成分分析（PCA）成功结合使用，以区分抗辐射（LY-R）和辐射敏感（LY-S）的小鼠淋巴瘤亚系（L5178Y）。正常拉曼分析的PCA结果显示，基于其特定的光谱指纹，抗辐射和辐射敏感细胞系之间存在差异。在使用金纳米颗粒（AuNPs）的SERS情况下，与辐射敏感亚系相比，在抗辐射亚系中观察到更大的光谱增强，这表明每个亚系与AuNPs表现出不同的相互作用。我们的结果表明，光谱和化学计量技术可作为预测淋巴瘤样本内在放射敏感性的补充工具。
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