Holtug K, Rasmussen H S, Mortensen P B
Department of Medicine, Hvidovre Hospital, University of Copenhagen, Denmark.
Scand J Clin Lab Invest. 1988 Nov;48(7):667-71.
An in vitro faecal incubation system was used to investigate how blood added to faeces influences short chain fatty acid (SCFA) production. The result was a change in SCFA pattern from one largely dominated by acetate and propionate to a pattern less dominated by these two acids but with greater amounts of longer and branched SCFA (butyrate, isobutyrate, valerate and isovalerate). Patients with active ulcerative colitis revealed variable concentrations of SCFA in their individual stool specimens, 66% of the samples being outside the 95% confidence interval set by a control group and without any specific trend. The SCFA concentrations were normal in patients with Crohn's disease of the colon. The study concludes that the changes in SCFA pattern seen elsewhere in studies on ulcerative colitis could be due to bacterial fermentation of blood either in the colon or in the stools after passing. It cautions against using faecal concentrations in this disease without due regard to the phenomenon of dilution or pollution of the colonic chymus by colonic effusion of blood.
采用体外粪便培养系统来研究向粪便中添加血液如何影响短链脂肪酸（SCFA）的产生。结果是SCFA模式发生了变化，从主要由乙酸盐和丙酸盐主导的模式转变为较少受这两种酸主导，但含有更多长链和支链SCFA（丁酸盐、异丁酸盐、戊酸盐和异戊酸盐）的模式。活动性溃疡性结肠炎患者的个体粪便样本中SCFA浓度各不相同，66%的样本超出了对照组设定的95%置信区间，且无任何特定趋势。结肠克罗恩病患者的SCFA浓度正常。该研究得出结论，在溃疡性结肠炎其他研究中观察到的SCFA模式变化可能是由于结肠中或粪便排出后血液的细菌发酵所致。研究告诫，在该疾病中使用粪便浓度时，应充分考虑结肠内容物被血液结肠渗出液稀释或污染的现象。
