从人胎盘中克隆出两种类型的GAP互补DNA。

Molecular cloning of two types of GAP complementary DNA from human placenta.

作者信息

Trahey M, Wong G, Halenbeck R, Rubinfeld B, Martin G A, Ladner M, Long C M, Crosier W J, Watt K, Koths K

机构信息

Department of Molecular Biology, Cetus Corp., Emeryville, CA 94608.

出版信息

Science. 1988 Dec 23;242(4886):1697-700. doi: 10.1126/science.3201259.

DOI:10.1126/science.3201259

PMID:3201259

Abstract

The ras p21 GTPase-activating protein (GAP) was purified from human placental tissue. Internal amino acid sequence was obtained from this 120,000-dalton protein and, by means of this sequence, two types of complementary DNA clones were isolated and characterized. One type encoded GAP with a predicted molecular mass of 116,000 daltons and 96% identity with bovine GAP. The messenger RNA of this GAP was detected in human lung, brain, liver, leukocytes, and placenta. The second type appeared to be generated by a differential splicing mechanism and encoded a novel form of GAP with a predicted molecular mass of 100,400 daltons. This protein lacks the hydrophobic amino terminus characteristic of the larger species, but retains GAP activity. The messenger RNA of this type was abundantly expressed in placenta and in several human cell lines, but not in adult tissues.

摘要

ras p21 GTP酶激活蛋白（GAP）是从人胎盘组织中纯化出来的。从这种120,000道尔顿的蛋白质中获得了内部氨基酸序列，并借助该序列分离和鉴定了两种互补DNA克隆。其中一种类型编码的GAP预测分子量为116,000道尔顿，与牛GAP有96%的同一性。这种GAP的信使核糖核酸在人肺、脑、肝、白细胞和胎盘中被检测到。第二种类型似乎是由差异剪接机制产生的，编码一种预测分子量为100,400道尔顿的新型GAP。这种蛋白质缺乏较大类型特有的疏水氨基末端，但保留了GAP活性。这种类型的信使核糖核酸在胎盘和几种人类细胞系中大量表达，但在成人组织中不表达。