Department of Oncology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Eur Rev Med Pharmacol Sci. 2020 Jan;24(2):704-711. doi: 10.26355/eurrev_202001_20049.
The aim of this study was to elucidate the function of long non-coding ribonucleic acids (lncRNAs) HANR in aggravating non-small cell lung cancer (NSCLC) progression via targeting microRNA-140-5p (miRNA-140-5p).
The relative expression level of HANR in NSCLC tissues and cell lines was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The correlation between HANR expression and the prognosis of NSCLC was analyzed. The regulatory effects of HANR on cellular behaviors of NSCLC cells were evaluated by Cell Counting Kit-8 (CCK-8), transwell and wound healing assay. Meanwhile, the relative expression of miRNA-140-5p in NSCLC tissues and cell lines was determined by qRT-PCR. In addition, rescue experiments were carried out to evaluate the potential influence of HANR/miRNA-140-5p on the progression of NSCLC.
HANR expression was significantly up-regulated in NSCLC tissues and cell lines. HANR expression was positively correlated with lymphatic metastasis and distant metastasis of NSCLC patients, whereas it was negatively correlated with the overall survival of NSCLC patients. Knockdown of HANR markedly suppressed the proliferative, migratory and invasive abilities of NSCLC cells. In NSCLC tissues, the miRNA-140-5p level was negatively associated with HANR level. Furthermore, inhibited cellular behaviors of NSCLC cells transfected with sh-HANR were partially reversed after miRNA-140-5p knockdown.
LncRNA HANR accelerates the proliferative, migratory and invasive abilities of NSCLC via negatively mediating miRNA-140-5p. Furthermore, HANR is closely correlated with lymphatic metastasis, distant metastasis and poor prognosis of NSCLC.
本研究旨在阐明长链非编码核糖核酸（lncRNA）HANR 通过靶向 microRNA-140-5p（miRNA-140-5p）在加重非小细胞肺癌（NSCLC）进展中的作用。
通过实时定量聚合酶链反应（qRT-PCR）测定 NSCLC 组织和细胞系中 HANR 的相对表达水平。分析 HANR 表达与 NSCLC 预后的相关性。通过细胞计数试剂盒-8（CCK-8）、Transwell 和划痕愈合试验评估 HANR 对 NSCLC 细胞细胞行为的调节作用。同时，通过 qRT-PCR 测定 NSCLC 组织和细胞系中 miRNA-140-5p 的相对表达。此外，进行了挽救实验，以评估 HANR/miRNA-140-5p 对 NSCLC 进展的潜在影响。
HANR 在 NSCLC 组织和细胞系中表达显著上调。HANR 表达与 NSCLC 患者的淋巴转移和远处转移呈正相关，而与 NSCLC 患者的总生存率呈负相关。敲低 HANR 显著抑制 NSCLC 细胞的增殖、迁移和侵袭能力。在 NSCLC 组织中，miRNA-140-5p 水平与 HANR 水平呈负相关。此外，miRNA-140-5p 敲低后，转染 sh-HANR 的 NSCLC 细胞的细胞行为部分逆转。
LncRNA HANR 通过负调控 miRNA-140-5p 加速 NSCLC 的增殖、迁移和侵袭能力。此外，HANR 与 NSCLC 的淋巴转移、远处转移和不良预后密切相关。
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