Zoo and Wildlife Veterinary Consultancy, 104 N Maleny Rd., North Maleny, Queensland 4552, Australia.
Fenner School of Environment and Society, The Australian National University, Building 141, Linnaeus Way, Canberra, Australian Capital Territory 2601, Australia.
J Wildl Dis. 2020 Jul;56(3):547-559. doi: 10.7589/2019-05-120. Epub 2020 Feb 4.
We evaluated the health of 31 (eight males, 23 females) founder eastern quolls (), translocated to a fenced reserve in the Australian Capital Territory between February 2016 and July 2017. Quolls were wild caught in Tasmania (16 animals) or captive bred at Mount Rothwell Biodiversity Interpretation Centre, Victoria (15 animals). Quolls were assessed for the presence of selected potential pathogens (, herpesviruses, serovars, hemoprotozoa, and ectoparasites). We assessed the relationships among sex, provenance (captive or free ranging), or herpesvirus infection, weight, and hematologic and biochemical variables. Six of 21 quolls (29%) tested were seropositive for antibodies to . Seropositive quolls weighed significantly more and had significantly lower potassium levels, anion gaps, and urea and triglyceride levels than seronegative quolls had. Eighteen of 31 (58%) combined conjunctival-pharyngeal-cloacal swabs collected from quolls were PCR positive for a newly identified gammaherpesvirus, tentatively named . There were no significant differences among hematologic and biochemical variables or body weights from PCR-positive and PCR-negative quolls. Eighteen of 18 (100%) of rectal-swab samples were culture negative for serovars. Three species of tick (, , and ), two species of mite (, one unidentified), and four species of flea (, , , and ), were detected on wild-caught quolls, whereas a fifth species of flea, , was detected only on captive-bred quolls. Five of 15 blood samples (33%) were positive for hemoprotozoan DNA via PCR, a novel species, a novel species, , and were detected. Despite the presence of several potential pathogens known to be associated with disease in other marsupials, the quolls were considered to be in good general health, suitable for translocation, and a viable population was subsequently established.
我们评估了 31 只（8 雄，23 雌）东部袋獾（）的健康状况，这些袋獾于 2016 年 2 月至 2017 年 7 月被转移到澳大利亚首都领地的一个围栏保护区。袋獾是在塔斯马尼亚（16 只动物）或维多利亚州罗斯韦尔生物多样性解读中心（15 只动物）野生捕获或圈养繁殖的。我们评估了选定的潜在病原体（疱疹病毒、血清型、血液原生动物和外寄生虫）的存在情况。我们评估了性别、起源（圈养或自由放养）、疱疹病毒感染与体重以及血液学和生物化学变量之间的关系。21 只袋獾中有 6 只（29%）的血清抗体检测呈阳性。血清阳性袋獾的体重显著更高，钾水平、阴离子间隙、尿素和甘油三酯水平显著更低。从袋獾采集的 31 只（58%）联合结膜-咽-泄殖腔拭子的 18 只 PCR 检测为一种新鉴定的γ疱疹病毒阳性，暂定名为。PCR 阳性和 PCR 阴性袋獾的血液学和生物化学变量或体重无显著差异。18 只直肠拭子样本的培养均为 血清型阴性。在野生袋獾上检测到三种蜱（、和）、两种螨（和一种未鉴定的）和四种蚤（、、和），而第五种蚤仅在圈养袋獾上检测到。通过 PCR，在 15 份血样中有 5 份（33%）为血液原生动物 DNA 阳性，检测到一种新型、一种新型、、和。尽管存在几种已知与其他有袋动物疾病相关的潜在病原体，但袋獾被认为总体健康状况良好，适合转移，随后建立了一个可行的种群。
