鸢尾素外周亚急性治疗对海马树突形成及星形胶质细胞分泌因子的影响。
The effects of peripherally-subacute treatment with irisin on hippocampal dendritogenesis and astrocyte-secreted factors.
作者信息
Kim Mun-Hee, Leem Yea-Hyun
出版信息
J Exerc Nutrition Biochem. 2019 Dec 31;23(4):32-35. doi: 10.20463/jenb.2019.0029.
PURPOSE
Fibronectin type III domain containing 5 (FNDC5)/irisin is an exercise-induced myokine, which contributes to cognitive functions. However, the relationship between the neuroprotective effects of FNDC5/irisin and hippocampal dendritic remodeling and astrocyte-secreted factors remains unclear. Therefore, we explored whether subchronic recombinant irisin treatment affected hippocampal morphology and some astrocyte-derived molecules.
METHODS
Mice were intraperitoneally injected with irisin (0.5 μg/kg/day) for seven days, followed by their sacrifice two days later. Hippocampal morphometric parameters were analyzed and pgc-1a, fndc5, bdnf, and some astrocyte-derived factors mRNA levels were measured.
RESULTS
Dendritic length, arborization, and spine density were enhanced by irisin regimen in hippocampal CA1 and CA3 areas. Hippocampal pgc-1a, fndc5, and bdnf mRNA levels were significantly increased by irisin treatment. Moreover, hevin mRNA levels were significantly enhanced, whereas tgf-b1 levels downregulated by irisin treatment.
CONCLUSION
FNDC5/irisin has dendritogenic activity probably through hevin induction and TGF-β1 suppression.
目的
含III型纤连蛋白结构域5（FNDC5）/鸢尾素是一种运动诱导的肌动蛋白，对认知功能有作用。然而，FNDC5/鸢尾素的神经保护作用与海马树突重塑和星形胶质细胞分泌因子之间的关系仍不清楚。因此，我们探讨了亚慢性重组鸢尾素治疗是否会影响海马形态和一些星形胶质细胞衍生分子。
方法
小鼠腹腔注射鸢尾素（0.5μg/kg/天），持续7天，两天后处死。分析海马形态计量学参数，并测量pgc-1a、fndc5、bdnf和一些星形胶质细胞衍生因子的mRNA水平。
结果
鸢尾素处理可增强海马CA1和CA3区的树突长度、分支和棘密度。鸢尾素处理显著提高了海马pgc-1a、fndc5和bdnf的mRNA水平。此外，鸢尾素处理使hevin mRNA水平显著升高，而tgf-b1水平下调。
结论
FNDC5/鸢尾素可能通过诱导hevin和抑制TGF-β1具有树突生成活性。
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