Faculty of Animal Science and Technology, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou 730070, China.
International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China.
Genes (Basel). 2020 Jan 30;11(2):148. doi: 10.3390/genes11020148.
Wool and hair fibres consist of a variety of proteins, including the keratin-associated proteins (KAPs). In this study, a putative ovine homologue of the human KAP21-2 gene () was identified. It was located on chromosome 1 as a 201-bp open reading frame (ORF) in the ovine genome assembly from a Texel sheep (v.4 NC_019458.2: nt122932727 to 122932927). A polymerase chain reaction- single strand conformation polymorphism (PCR-SSCP) analysis of this ORF, and subsequent DNA sequencing, identified five sequences (named -). The putative amino acid sequences that would be produced, shared some identity with each other and with other KAPs, but they were most similar to ovine KAP21-1, and phylogenetically related to human KAP21-2. The location of the ovine sequence was consistent with the location of human and this suggests they represent different variant forms of ovine -2. Variation in this gene was investigated in 389 Merino (sire) × Southdown-cross (ewe) lambs. These were derived from four independent sire-lines. The sequence variation was found to be associated with variation in five wool traits: including mean staple length (MSL), mean fibre diameter (MFD), fibre diameter standard deviation (FDSD), prickle factor (PF), and greasy fleece weight (GFW). The most persistent effect of variation was with variation in MSL; with the MSL of sheep of genotype being 12.5% greater than those of genotype . A similar effect was observed from individual variant absence/presence models. This suggests that should be further investigated as a possible gene-marker for improving MSL.
羊毛和毛发纤维由多种蛋白质组成，包括角蛋白相关蛋白（KAP）。在这项研究中，鉴定出了一种假定的绵羊 KAP21-2 基因（）的同源物。它位于 1 号染色体上，在特克赛尔绵羊（v.4 NC_019458.2：nt122932727 到 122932927）的绵羊基因组组装中为 201bp 的开放阅读框（ORF）。对该 ORF 的聚合酶链反应-单链构象多态性（PCR-SSCP）分析，以及随后的 DNA 测序，鉴定了五个序列（命名为 -）。推测产生的氨基酸序列彼此之间以及与其他 KAP 具有一定的同源性，但与绵羊 KAP21-1 最为相似，并且在系统发育上与人类 KAP21-2 相关。绵羊 序列的位置与人类 序列的位置一致，这表明它们代表不同的绵羊 -2 变体形式。在 389 只美利奴（父本）×萨福克杂交（母本）羔羊中研究了该基因的变异情况。这些羔羊来自四个独立的父本系。序列变异与五个羊毛性状的变异有关：包括平均纤维长度（MSL）、平均纤维直径（MFD）、纤维直径标准差（FDSD）、刺毛因子（PF）和油腻羊毛重量（GFW）。 变异与 MSL 变异的关系最为持久；基因型 羊的 MSL 比基因型 羊长 12.5%。从个体变体缺失/存在模型中也观察到了类似的效果。这表明 应该进一步研究，作为提高 MSL 的可能基因标记。
