Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University of Vienna, Vienna, Austria.
Department of Pharmacognosy, University of Vienna, Vienna, Austria.
Sci Rep. 2020 Feb 4;10(1):1788. doi: 10.1038/s41598-020-58391-y.
The preferable route for treatment of peritoneal dialysis related peritonitis remains the intraperitoneal administration of antibiotics admixed to peritoneal dialysis fluids. It is important to know whether the administered drug is compatible with the PD fluids and its container. In the present study the compatibility of aztreonam with four commercial PDFs at storing temperatures and duration representative for storing conditions in the clinical settings was investigated. Aztreonam concentrations were determined using high-performance liquid chromatography. The antimicrobial activity of aztreonam was evaluated using an E. coli diffusion disk inhibition assay and P. aeruginosa time-kill curves. In Extraneal evaluated at 6 °C, 25 °C and 37 °C aztreonam was stable over the whole study period of 14 days and 24 hours, respectively. In Physioneal and Nutrineal aztreonam was stable at 6 °C for up to 14 days. Antimicrobial activity was retained in all PD fluids over the whole study period. Aztreonam remained stable and was compatible with the PD fluids, particularly with Extraneal or Nutrineal, and no compensatory dose adjustment is needed when stored for up to 14 days at refrigeration temperature before use.
治疗腹膜透析相关性腹膜炎的首选方法仍是将抗生素混入腹膜透析液中进行腹腔内给药。了解所给予的药物是否与 PD 液及其容器相容非常重要。在本研究中，考察了在储存温度和持续时间（代表临床环境中的储存条件）下，氨曲南与四种商业 PD 液的相容性。使用高效液相色谱法测定氨曲南的浓度。采用大肠杆菌扩散盘抑制试验和铜绿假单胞菌时间杀伤曲线评估氨曲南的抗菌活性。在 Extraneal 分别于 6°C、25°C 和 37°C 下评估，氨曲南在整个 14 天和 24 小时的研究期间均稳定。在 Physioneal 和 Nutrineal 中，氨曲南在 6°C 下可稳定 14 天。在整个研究期间，所有 PD 液中的抗菌活性均得以保留。氨曲南在 PD 液中稳定，尤其是与 Extraneal 或 Nutrineal 相容，在冷藏温度下储存不超过 14 天，使用前无需进行补偿性剂量调整。
