Click Eleanor S, Finlay Alyssa, Oeltmann John E, Basotli Joyce, Modongo Chawangwa, Boyd Rosanna, Wen Xiao Jun, Shepherd James, Moonan Patrick K, Zetola Nicola M
Division of Global HIV and TB, Centers for Disease Control and Prevention, Atlanta, GA, United States of America.
TB/HIV Research, Centers for Disease Control and Prevention, Gaborone, Botswana; Division of Tuberculosis Elimination, Centers for Disease Control and Prevention, Division of Tuberculosis Elimination, Atlanta, GA, USA.
Infect Genet Evol. 2020 Jul;81:104232. doi: 10.1016/j.meegid.2020.104232. Epub 2020 Feb 3.
Mycobacterium tuberculosis complex (MTBC) is divided into several major phylogenetic lineages, with differential distribution globally. Using population-based data collected over a three year period, we performed 24-locus Mycobacterial Interspersed Repeat Unit - Variable Number Tandem Repeat (MIRU-VNTR) genotyping on all culture isolates from two districts of the country that differ in tuberculosis (TB) incidence (Gaborone, the capital, and Ghanzi in the Western Kalahari). The study objective was to characterize the molecular epidemiology of TB in these districts. Overall phylogenetic diversity mirrored that reported from neighboring Republic of South Africa, but differences in the two districts were marked. All four major lineages of M. tuberculosis were found in Gaborone, but only three of the four major lineages were found in Ghanzi. Strain diversity was lower in Ghanzi, with a large proportion (38%) of all isolates having an identical MIRU-VNTR result, compared to 6% of all isolates in Gaborone with the same MIRU-VNTR result. This study demonstrates localized differences in strain diversity by two districts in Botswana, and contributes to a growing characterization of MTBC diversity globally.
结核分枝杆菌复合群（MTBC）分为几个主要的系统发育谱系，在全球分布存在差异。利用三年期间收集的基于人群的数据，我们对该国两个结核病（TB）发病率不同的地区（首都哈博罗内和西喀拉哈里的杭济）的所有培养分离株进行了24位点分枝杆菌间插重复单元-可变数目串联重复序列（MIRU-VNTR）基因分型。研究目的是描述这些地区结核病的分子流行病学特征。总体系统发育多样性与邻国南非报告的情况相似，但两个地区存在明显差异。在哈博罗内发现了结核分枝杆菌的所有四个主要谱系，但在杭济仅发现了四个主要谱系中的三个。杭济的菌株多样性较低，所有分离株中有很大比例（38%）的MIRU-VNTR结果相同，而在哈博罗内所有分离株中这一比例为6%。这项研究证明了博茨瓦纳两个地区菌株多样性的局部差异，并有助于全球范围内对MTBC多样性的不断深入了解。
