University of Agriculture and Forestry, Hue University, Hue, Vietnam.
Institute of Aquaculture, University of Stirling, Stirling, UK.
J Fish Dis. 2020 Mar;43(3):371-378. doi: 10.1111/jfd.13135.
A bacterial infectivity challenge model of Edwardsiella ictaluri in striped catfish was developed. All experiments were conducted using a bacterial isolate of E. ictaluri that had been recovered during a natural outbreak of bacillary necrosis of Pangasianodon (BNP) in farmed striped catfish Pangasianodon hypophthalmus in Vietnam. Time of immersion in 10 CFU.ml had a significant effect on mortality. The immersion bacterial dose of 10 CFU/ml for 30 s resulted in a cumulative percentage mortality of 63%. Three to four days post-bacterial challenge, fish showed gross clinical signs of natural BNP and E. ictaluri was recovered and identified from these fish. Moreover, a cohabitation challenge was evaluated as an alternative challenge method, although the mortalities among the infected fish were lower at around 15%-40%. This study confirmed the horizontal transmission of E. ictaluri in striped catfish and elucidated that cohabitation challenge could be used in reproducing the disease under controlled conditions.
建立了斑点叉尾鮰爱德华氏菌的细菌性感染挑战模型。所有实验均使用从越南养殖斑点叉尾鮰细菌性坏死病（BNP）自然暴发中分离的爱德华氏菌细菌分离株进行。在 10 CFU.ml 中的浸泡时间对死亡率有显著影响。在 10 CFU/ml 的细菌浸泡 30 秒导致累积死亡率为 63%。细菌攻毒后 3 至 4 天，鱼出现了天然 BNP 的明显临床症状，并且从这些鱼中回收并鉴定出了爱德华氏菌。此外，评估了共栖攻毒作为替代攻毒方法，尽管感染鱼的死亡率约为 15%-40%。本研究证实了爱德华氏菌在斑点叉尾鮰中的水平传播，并阐明了共栖攻毒可用于在受控条件下再现疾病。
