Life Sciences Institute, Department of Medicinal Chemistry, The University of Michigan, USA.
Life Science Institute, The University of Michigan, USA.
Angew Chem Int Ed Engl. 2020 May 18;59(21):8166-8172. doi: 10.1002/anie.201913686. Epub 2020 Mar 19.
Stereospecific polycyclic core formation of hapalindoles and fischerindoles is controlled by Stig cyclases through a three-step cascade involving Cope rearrangement, 6-exo-trig cyclization, and a final electrophilic aromatic substitution. Reported here is a comprehensive study of all currently annotated Stig cyclases, revealing that these proteins can assemble into heteromeric complexes, induced by Ca , to cooperatively control the stereochemistry of hapalindole natural products.
通过涉及 Cope 重排、6-endo-trig 环化和最终亲电芳香取代的三步级联反应，Stig 环化酶控制了 hapalindoles 和 fischerindoles 的立体特异性多环核心形成。本文全面研究了所有现有注释的 Stig 环化酶，揭示了这些蛋白可以组装成异源复合物，由 Ca 诱导，协同控制 hapalindole 天然产物的立体化学。
