Biochemistry Department, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil.
Biochemistry Department, Federal University of Santa Catarina, 88040-900, Florianopolis, SC, Brazil.
Mar Environ Res. 2020 Feb;154:104870. doi: 10.1016/j.marenvres.2019.104870. Epub 2019 Dec 26.
Glutathione (GSH) is a major cellular antioxidant molecule participating in several biological processes, including immune function. In this study, we investigated the importance of GSH to oysters Crassostrea gigas immune response. Oysters were treated with the GSH-synthesis inhibitor buthionine sulfoximine (BSO), and the function of immune cells and mortality were evaluated after a bacterial challenge with different Vibrio species. BSO caused a moderate decrease (20-40%) in GSH levels in the gills, digestive gland, and hemocytes. As expected, lower GSH decreased survival to peroxide exposure. Hemocyte function was preserved after BSO treatment, however, oysters became more susceptible to challenges with Vibrio anguillarum, V. alginolyticus, or V. harveyi, but not with V. parahaemolyticus and V. vulnificus, indicating a species-specific vulnerability. Our study indicates that in natural habitats or in mariculture farms, disturbances in GSH metabolism may pre-dispose oysters to bacterial infection, decreasing survival.
谷胱甘肽（GSH）是一种重要的细胞抗氧化分子，参与多种生物学过程，包括免疫功能。在这项研究中，我们研究了 GSH 对牡蛎 Crassostrea gigas 免疫反应的重要性。用 GSH 合成抑制剂丁硫氨酸亚砜胺（BSO）处理牡蛎，并用不同的弧菌属细菌对其进行细菌挑战后，评估免疫细胞的功能和死亡率。BSO 导致鳃、消化腺和血细胞中 GSH 水平适度下降（20-40%）。正如预期的那样，较低的 GSH 会降低对过氧化物暴露的存活率。然而，BSO 处理后保持了血细胞功能，但牡蛎对鳗弧菌、溶藻弧菌或哈维氏弧菌的挑战变得更加敏感，但对副溶血性弧菌和创伤弧菌则不敏感，表明存在种特异性脆弱性。我们的研究表明，在自然栖息地或海水养殖农场中，GSH 代谢的干扰可能使牡蛎易受细菌感染，降低存活率。
