dePolo Gwen E, Schafer Emily, Sadman Kazi, Rivnay Jonathan, Shull Kenneth R
Department of Materials Science and Engineering, Northwestern University.
Department of Biomedical Engineering, Northwestern University.
J Vis Exp. 2020 Jan 22(155). doi: 10.3791/60584.
In this study, we present various examples of how thin film preparation for quartz crystal microbalance experiments informs the appropriate modeling of the data and determines which properties of the film can be quantified. The quartz crystal microbalance offers a uniquely sensitive platform for measuring fine changes in mass and/or mechanical properties of an applied film by observing the changes in mechanical resonance of a quartz crystal oscillating at high frequency. The advantages of this approach include its experimental versatility, ability to study changes in properties over a wide range of experimental time lengths, and the use of small sample sizes. We demonstrate that, based on the thickness and shear modulus of the layer deposited on the sensor, we can acquire different information from the material. Here, this concept is specifically exploited to display experimental parameters resulting in mass and viscoelastic calculations of adsorbed collagen on gold and polyelectrolyte complexes during swelling as a function of salt concentration.
在本研究中，我们展示了各种示例，说明用于石英晶体微天平实验的薄膜制备如何为数据的适当建模提供依据，并确定薄膜的哪些属性可以被量化。石英晶体微天平提供了一个极其灵敏的平台，通过观察高频振荡的石英晶体的机械共振变化来测量施加薄膜的质量和/或机械性能的细微变化。这种方法的优点包括其实验通用性、在广泛的实验时间长度范围内研究性能变化的能力以及使用小样本量。我们证明，基于沉积在传感器上的层的厚度和剪切模量，我们可以从材料中获取不同的信息。在此，这一概念被专门用于展示实验参数，这些参数导致在溶胀过程中根据盐浓度对吸附在金和聚电解质复合物上的胶原蛋白进行质量和粘弹性计算。
