Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences; Yunnan Provincial Key Lab of Agricultural Biotechnology; Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture. 2238# Beijing Rd, Wuhua Prefecture, Kunming 650205, Yunnan, P. R. China.
Department of Biotechnology, Asia University, Wufeng, Taichung 41354, Taiwan.
Plant Dis. 2020 Apr;104(4):1175-1182. doi: 10.1094/PDIS-09-19-1925-RE. Epub 2020 Feb 15.
Chilli pepper ( L.) is one of the most important crops in Yunnan Province, China. An orthotospovirus isolate 14YV855 was isolated from a diseased chilli pepper plant exhibiting yellow ringspots and necrosis on leaves in Shiping County, Honghe Hani and Yi Autonomous Prefecture, Yunnan Province in 2014. The complete genome sequence of 14YV855 was determined. The small, medium, and large RNAs are 3,428, 4,781, and 8,917 nucleotides long, respectively. The complete nucleocapsid (N) protein of 14YV855 shares a high amino acid identity of 84.8 to 89.9% to that of (CaCV), (GBNV), (WBNV), and (WSMoV), which is slightly less than the 90% identity threshold for the demarcation of new sp. Phylogenetic analyses revealed that the N protein and RNA-dependent RNA polymerase of 14YV855 are the most related to WSMoV, while the NSs, NSm, and Gn/Gc proteins are similar to those of GBNV. As expected, 14YV855 is serologically related to CaCV, GBNV, WBNV, and WSMoV when the monoclonal antibody against the N protein of WSMoV was used; however, 14YV855 can be distinguished from other orthotospoviruses by reverse-transcription PCR using the specific primers. Our results indicate that 14YV855 is a new sp. belonging to the WSMoV serogroup and is provisionally named Chilli yellow ringspot virus.
辣椒（L.）是中国云南省最重要的作物之一。2014 年，在云南省红河哈尼族彝族自治州石屏县，从一株表现出叶片黄色环斑和坏死的病辣椒植物中分离到一株辣椒黄环斑病毒 14YV855。测定了 14YV855 的全基因组序列。小、中、大 RNA 分别长 3428、4781 和 8917 个核苷酸。14YV855 的完整核衣壳（N）蛋白与（CaCV）、（GBNV）、（WBNV）和（WSMoV）的氨基酸同一性高达 84.8%至 89.9%，稍低于新种划分的 90%同一性阈值。系统进化分析表明，14YV855 的 N 蛋白和 RNA 依赖性 RNA 聚合酶与 WSMoV 最为相关，而 NSs、NSm 和 Gn/Gc 蛋白与 GBNV 相似。正如预期的那样，当使用针对 WSMoV N 蛋白的单克隆抗体时，14YV855 与 CaCV、GBNV、WBNV 和 WSMoV 在血清学上相关；然而，14YV855 可以通过使用特异性引物的逆转录 PCR 与其他正呼肠孤病毒区分开来。我们的结果表明，14YV855 是一种新的属于 WSMoV 血清群的病毒，暂命名为辣椒黄环斑病毒。
