采用新型超交联聚合物固相萃取结合高效液相色谱法灵敏测定豆浆和番茄样品中的苯脲类除草剂。
Sensitive determination of phenylurea herbicides in soybean milk and tomato samples by a novel hypercrosslinked polymer based solid-phase extraction coupled with high performance liquid chromatography.
机构信息
College of Science, Hebei Agricultural University, Baoding 071001, Hebei, China.
Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.
出版信息
Food Chem. 2020 Jul 1;317:126410. doi: 10.1016/j.foodchem.2020.126410. Epub 2020 Feb 15.
A heterocyclic hypercrosslinked polymer (Py-DMB HCP) was prepared by Friedel-Crafts alkylation reaction using pyrrole as monomer and p-dimethoxybenzene as crosslinker. The Py-DMB HCP was employed as an adsorbent for solid-phase extraction (SPE) of phenylurea herbicides (PUHs) and showed superior extraction performance. Thus, a sensitive analytical method for the determination of PUHs from soybean milk and tomato samples was established by combining Py-DMB HCP based SPE with high performance liquid chromatography. Under the optimal conditions, good sensitivity was achieved with the detection limits of 0.10-0.20 ng mL and 0.06-0.15 ng g for soybean milk and tomato samples, respectively. The linearity range of PUHs was 0.3-150.0 ng mL and 0.2-150.0 ng g, respectively. The recoveries were 87.4% to 116% with relative standard deviations lower than 6.5%. Density functional theory calculation was performed to study the intermolecular interaction mechanism, demonstrating the coexistence of both the H-bonding and π-π interactions in the adsorption process.
采用吡咯作为单体和对二甲氧基苯作为交联剂，通过傅克烷基化反应制备了杂环超交联聚合物（Py-DMB HCP）。Py-DMB HCP 用作固相萃取（SPE）的吸附剂，用于测定豆奶和番茄样品中的苯脲类除草剂（PUHs），具有优异的萃取性能。因此，通过将基于 Py-DMB HCP 的 SPE 与高效液相色谱法相结合，建立了从豆奶和番茄样品中测定苯脲类除草剂的灵敏分析方法。在最佳条件下，对豆奶和番茄样品的检测限分别为 0.10-0.20ng mL 和 0.06-0.15ng g，线性范围分别为 0.3-150.0ng mL 和 0.2-150.0ng g。PUHs 的回收率为 87.4%至 116%，相对标准偏差低于 6.5%。通过密度泛函理论计算研究了分子间相互作用机制，表明在吸附过程中同时存在氢键和π-π相互作用。
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