Shim Sun-Yup, Kim Ji-Hyun, Kho Kang-Hee, Lee Mina
Department of Aqualife Science, College of Fisheries and Ocean Sciences, Chonnam National University, 50, Daehak-Ro, Yeosu, Jeonanm 59626, Republic of Korea.
Department of Food and Nutrition, Sunchon National University, Suncheon, Jeonnam 57922, Republic of Korea.
Toxicol Rep. 2020 Jan 27;7:277-281. doi: 10.1016/j.toxrep.2020.01.018. eCollection 2020.
In this present study, we examined the anti-inflammatory and anti-oxidative properties of alcoholic lemon myrtle extract (LME). The total polyphenol and flavonoid content of LME were determined as 118.77 and 14.53 mg/g extract, respectively. LME showed anti-oxidative properties, such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) radical scavenging activity. The anti-inflammatory activities of LME were investigated using the lipopolysaccharide-stimulated murine macrophage RAW 264.7 cells. Pretreatment with LME was performed at non-cytotoxic concentrations of 10-100 μg/mL. LME inhibited the production of inflammatory mediators such as nitric oxide (NO). Enzyme-linked immunosorbent assay and reverse-transcriptase polymerase chain reaction (RT-PCR) revealed that pretreatment with LME suppressed the protein expression and mRNA levels of pro-inflammatory cytokines such as interleukin IL-6, and tumor necrosis factor (TNF)-α in a concentration-dependent manner, respectively. These results suggest that LME could be used as a potential therapeutic agent having potent anti-inflammatory effects that could be used to treat inflammatory bowel disease.
在本研究中，我们检测了柠檬桃金娘乙醇提取物（LME）的抗炎和抗氧化特性。LME的总多酚和黄酮含量分别测定为118.77和14.53mg/g提取物。LME表现出抗氧化特性，如对2,2-二苯基-1-苦基肼基水合物（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）的自由基清除活性。使用脂多糖刺激的小鼠巨噬细胞RAW 264.7细胞研究了LME的抗炎活性。在10-100μg/mL的无细胞毒性浓度下用LME进行预处理。LME抑制了一氧化氮（NO）等炎症介质的产生。酶联免疫吸附测定和逆转录聚合酶链反应（RT-PCR）表明，用LME预处理分别以浓度依赖的方式抑制了促炎细胞因子如白细胞介素IL-6和肿瘤坏死因子（TNF）-α的蛋白质表达和mRNA水平。这些结果表明，LME可作为一种具有强大抗炎作用的潜在治疗剂，可用于治疗炎症性肠病。
