通过31P磁共振波谱法测量脑葡萄糖摄取率。

A measurement of cerebral glucose uptake rate by 31P MRS.

作者信息

Koga K, Miura I

机构信息

Tokushima Res. Inst., Otsuka Pharmaceutical Co., Ltd, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Dec 30;157(3):1258-63. doi: 10.1016/s0006-291x(88)81010-6.

DOI:10.1016/s0006-291x(88)81010-6

PMID:3207425

Abstract

A method for the measurement of cerebral glucose uptake rate by in vivo 31P Magnetic Resonance Spectroscopy (MRS) is proposed. The initial rate of 2-deoxy-glucose (DG) uptake after DG administration is measured by the increase of 2-deoxy-glucose-6-phosphate (DG6P) signal at a chemical shift of 7.2 ppm with respect to phosphocreatine (PCr). The values for four different metabolic states of rat brain (two levels of epileptic seizures induced by bicuculline, nitrous oxide analgesia and pentobarbital anesthesia) were in good agreement with the previously reported ones by radioisotope methods. This method appears to be useful for measuring cerebral glucose uptake rate.

摘要

提出了一种通过体内31P磁共振波谱（MRS）测量脑葡萄糖摄取率的方法。在给予2-脱氧葡萄糖（DG）后，通过相对于磷酸肌酸（PCr）在化学位移7.2 ppm处2-脱氧葡萄糖-6-磷酸（DG6P）信号的增加来测量DG摄取的初始速率。大鼠脑四种不同代谢状态（荷包牡丹碱诱导的两种癫痫发作水平、一氧化二氮镇痛和戊巴比妥麻醉）的值与先前通过放射性同位素方法报道的值高度一致。该方法似乎可用于测量脑葡萄糖摄取率。