Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran.
Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran; Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Gene. 2020 May 15;738:144457. doi: 10.1016/j.gene.2020.144457. Epub 2020 Feb 17.
Breast cancer is caused by the interaction of inherited and environmental risk factors. Also, gastric cancer is the second fatal carcinoma. B-cell leukemia/lymphoma 2 gene family plays a crucial role in carcinogenesis by inhibiting the apoptosis process.
In this study, 129 patients with breast cancer and 132 controls as well as 136 patients with gastric cancer and 50 controls were enrolled. We used Real time PCR to determine the genotype of the samples. Finally, we analyzed the diagram based on high temperature melting curve diagram using the MICPCR software, followed by bioinformatics prediction of rs1016860 functions.
rs1016860 of BCL2 gene with CC, CT, and TT genotypes were observed in this region. The association of Estrogen receptor and Progesterone receptor, cancer stage and grade of cancer in the patients with genotypes was significant in breast cancer. The association of the status of primary tumor in the patients with genotypes is significant in gastric cancer (Chi-Square p < 0.05 and p = 0.000 did not follow the Hardy-Weinberg equilibrium).
It was predicted that the TT genotype could be dangerous in breast cancer and gastric cancer; it is expected via bioinformatics that this SNP could lead to signaling pathways of cancer progression, by altering the binding potential of miR-629-5p to BCL2 3'UTR.
乳腺癌是由遗传和环境风险因素相互作用引起的。此外，胃癌是第二致命的癌种。B 细胞白血病/淋巴瘤 2 基因家族通过抑制细胞凋亡过程在致癌作用中发挥关键作用。
本研究纳入了 129 名乳腺癌患者和 132 名对照者，以及 136 名胃癌患者和 50 名对照者。我们使用实时 PCR 确定了样本的基因型。最后，我们使用 MICPCR 软件根据高温融解曲线图分析了图谱，并对 rs1016860 的功能进行了生物信息学预测。
在该区域观察到 BCL2 基因的 rs1016860 具有 CC、CT 和 TT 基因型。在乳腺癌患者中，基因型与雌激素受体和孕激素受体、癌症分期和癌症分级有关。在胃癌患者中，基因型与原发性肿瘤状态有关（卡方检验 p < 0.05，p = 0.000 未遵循哈迪-温伯格平衡）。
通过生物信息学预测，TT 基因型可能在乳腺癌和胃癌中具有危险性，这可能导致 miR-629-5p 与 BCL2 3'UTR 的结合潜力发生改变，从而改变癌症进展的信号通路。
