Chemical Engineering and Applied Chemistry, Energy and Bioproducts Research Institute, Aston University, B4 7ET, Birmingham, United Kingdom.
Plant Physiol Biochem. 2020 Apr;149:256-265. doi: 10.1016/j.plaphy.2020.02.019. Epub 2020 Feb 15.
Chemical stimulants, used to enhance biomass yield, are highly desirable for the commercialisation of algal products for a wide range of applications in the food, pharma and biofuels sectors. In the present study, phenolic compounds, varying in substituents and positional isomers on the arene ring have been evaluated to determine structure-activity relationship and growth. The phenols, catechol, 4-methylcatechol and 2, 4-dimethyl phenol were generally inhibitory to growth as were the compounds containing an aldehyde function. By contrast, the phenolic acids, salicylic acid, aspirin and 4-hydroxybenzoate markedly stimulated cell proliferation enhancing cell numbers by 20-45% at mid-log phase. The order of growth stimulation was ortho > para > meta with respect to the position of the OH group. Both SA and aspirin reduced 16:3 in chloroplast galactolipids. In addition, both compounds inhibited lipoxygenase activity and lowered the levels of lipid hydroperoxides and malondialdehydes in the cells. The present study has demonstrated the possibility of using SA or aspirin to promote algal growth through the manipulation of lipid metabolising enzymes.
化学刺激物，用于提高生物量产量，对于藻类产品的商业化应用具有重要意义，这些产品可应用于食品、制药和生物燃料等多个领域。在本研究中，评估了具有不同取代基和芳环位置异构体的酚类化合物，以确定结构-活性关系和生长情况。儿茶酚、4-甲基儿茶酚和 2,4-二甲基苯酚等酚类化合物通常对生长具有抑制作用，而含有醛基的化合物也是如此。相比之下，酚酸如水杨酸、阿司匹林和 4-羟基苯甲酸则明显刺激细胞增殖，在对数中期将细胞数量提高了 20-45%。就 OH 基团的位置而言，生长刺激的顺序为邻位＞对位＞间位。SA 和阿司匹林均降低了叶绿体半乳糖脂中的 16:3。此外，这两种化合物均抑制脂氧合酶的活性，降低细胞中脂质过氧化物和丙二醛的水平。本研究表明，通过操纵脂质代谢酶，使用 SA 或阿司匹林促进藻类生长是可能的。
