College of Life Science, Yantai University, Yantai, P. R. China.
College of Life Sciences, China Jiliang University, Hangzhou, P. R. China.
J Sep Sci. 2020 Jun;43(11):2133-2141. doi: 10.1002/jssc.201901261. Epub 2020 Mar 18.
Molecularly imprinted polymers for strobilurin fungicides were prepared by precipitation polymerization employing azoxystrobin as template molecular together with methacrylic acid monomer and trimethylolpropane triacrylate cross-linker. Morphological characterization showed molecularly imprinted polymers were uniform spherical particles with about 0.2 μm in diameter, while the morphologies of nonimprinted polymers were irregular bulk. The equilibrium binding and selective experiments proved that molecularly imprinted polymers possessed a higher affinity toward four fungicides compared to nonimprinted polymers and heterogeneous binding sites were found in the molecularly imprinted polymers. Molecularly imprinted solid-phase extraction conditions, including sample loading solvents, selective washing, and elution solvents, were carefully optimized. The developed method showed good recoveries (70.0-114.0%) with relative standard deviations in range of 1.0-9.8% (n = 3) for samples (cucumber and peach) spiked at three different levels (10, 50, and 100 μg/ kg). The detection limit (signal/noise = 3) ranged from 0.01 to 0.08 μg/kg. The results demonstrated good potential use of this convenient and highly efficient method for determining trace strobilurin fungicides in agricultural products.
采用沉淀聚合法，以嘧菌酯为模板分子，与甲基丙烯酸单体和三羟甲基丙烷三丙烯酸酯交联剂一起，制备了用于防治苯并咪唑类杀菌剂的分子印迹聚合物。形态学特征表明，分子印迹聚合物为均匀的球形颗粒，直径约为 0.2μm，而非印迹聚合物的形态不规则块状。平衡结合和选择性实验证明，与非印迹聚合物相比，分子印迹聚合物对四种杀菌剂具有更高的亲和力，并且在分子印迹聚合物中发现了异构结合位点。仔细优化了分子印迹固相萃取条件，包括样品加载溶剂、选择性洗涤和洗脱溶剂。该方法对黄瓜和桃样品（在三个不同水平（10、50 和 100μg/kg）下进行了加标）的回收率良好（70.0-114.0%），相对标准偏差在 1.0-9.8%（n=3）范围内。检测限（信噪比=3）范围为 0.01-0.08μg/kg。结果表明，该简便高效的方法在测定农产品中痕量苯并咪唑类杀菌剂方面具有良好的应用潜力。
