Department of Biochemistry, Veterinary Faculty, Harran University, 63200, Şanlıurfa, Turkey.
Department of Pharmacy Services, Vocational School of Health Services, Harran University, 63200, Şanlıurfa, Turkey.
Appl Biochem Biotechnol. 2020 Aug;191(4):1353-1368. doi: 10.1007/s12010-020-03287-w. Epub 2020 Feb 26.
Oxidative stress can play an important role in the autoxidation of glucose, glycation of proteins and activation of polyol metabolism, and the development of various complications in diabetes. This study investigated the protective effect of Paliurus spina-christi (PSC) fruit against diabetes-induced pathologies such as inflammation caused by oxidative stress and its phytochemical content. The bioactive compounds of Paliurus spina-christi fruit extract (PSC-FE) prepared by the infusion method were determined by LC-MS/MS analysis, and the metal reduction and radical removal activity were determined in vitro by DPPH, ABTS, FRAP and CUPRAC methods. The effect of PSC-FE on catalase and superoxide dismutase enzymes and lipid peroxidation in streptozotocin-induced diabetic rat tissues was determined in vivo. For determining in vivo antioxidant activity, the study was carried out on 30 Wistar albino male rats that were distributed into 3 research and 2 control groups. As a result of LC-MS/MS analysis, malic acid (28,424.18 ± 3.21 μg/g PSC-FE) and rutin (23,459.68 ± 3.73 μg/g PSC-FE) are the highest among the 22 phenolic compounds. The PSC fruit water extracts were determined to have a more powerful antioxidant activity than standard antioxidants. In those groups that received PSC-FE, catalase and superoxide dismutase enzyme levels that decreased in patient group were found to have a statistically meaningful increase in treatment groups (P < 0.01). Although malondialdehyde amount, the last product of lipid peroxidation, was observed to have a statistically meaningful increase in patient group when compared with control group, this increase was decreased in all treatment groups in a statistically meaningful manner (P < 0.01). The PSC was seen to be more successful, when compared with standard antioxidants. The PSC-FE was found to have a rich phenolic diversity. It can be said that the PSC may have a significant therapeutic effect on diabetes caused by oxidative stress due to its biological activities and content.
氧化应激在葡萄糖的自动氧化、蛋白质的糖化和多元醇代谢的激活以及糖尿病的各种并发症的发展中起着重要作用。本研究调查了火棘（Paliurus spina-christi）果实对糖尿病引起的病理的保护作用，如氧化应激引起的炎症及其植物化学物质含量。采用 LC-MS/MS 分析方法测定浸渍法制备的火棘果实提取物（PSC-FE）中的生物活性化合物，采用 DPPH、ABTS、FRAP 和 CUPRAC 方法测定其体外金属还原和自由基清除活性。体内研究了 PSC-FE 对链脲佐菌素诱导的糖尿病大鼠组织中过氧化氢酶和超氧化物歧化酶酶及脂质过氧化的影响。为了确定体内抗氧化活性，在 30 只 Wistar 白化雄性大鼠上进行了这项研究，这些大鼠被分为 3 个研究组和 2 个对照组。通过 LC-MS/MS 分析，在 22 种酚类化合物中，苹果酸（28,424.18±3.21μg/g PSC-FE）和芦丁（23,459.68±3.73μg/g PSC-FE）的含量最高。与标准抗氧化剂相比，火棘果实水提取物的抗氧化活性更强。在接受 PSC-FE 的各组中，与患者组相比，在治疗组中观察到过氧化氢酶和超氧化物歧化酶酶水平显著增加（P<0.01）。虽然与对照组相比，患者组丙二醛（脂质过氧化的最终产物）的含量显著增加，但在所有治疗组中，这种增加均呈统计学意义的降低（P<0.01）。与标准抗氧化剂相比，火棘更为成功。PSC-FE 被发现具有丰富的酚类多样性。可以说，由于其生物活性和含量，火棘可能对氧化应激引起的糖尿病具有显著的治疗作用。
