Liu W, Tan L, Huang Y, Li W C, Liu Y S, Yang L C
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China.
Hunan Co-Innovation Center of Animal Production Safety, Changsha, China.
J Helminthol. 2020 Feb 27;94:e131. doi: 10.1017/S0022149X20000139.
Sparganosis is an important foodborne parasitic zoonosis; however, few reports on the prevalence of snake-infecting plerocercoids from Hunan province in China are available. Therefore, we investigated the prevalence of spargana infection in wild snakes from this region in 2018, and identified an astonishing prevalence rate of 91.7% (344/375). Spargana parasites were found in 99.1% of Zaocys dhumnades, 94.1% of Elaphe carinata and 86.7% of Elaphe taeniura. Parasites exhibited various distributions: 50% were located in muscular tissue, 32.1% in subcutaneous tissue and 17.9% in the coelomic cavity. To identify the specific status of spargana collected from wild snakes, partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequences were amplified, sequenced and analysed. Sequence variations for cox1 among all the examined plerocercoids ranged between 0.0 and 2.9%, with 21 variable sites identified (4.71%, 21/446). Phylogenetic analyses identified that all plerocercoids isolated from Hunan province were Spirometra erinaceieuropaei. This is the first report of S. erinaceieuropaei infection in snakes in Hunan province. The risks and harms of sparganosis should be publicized, and illegal wildlife trade should be controlled.
裂头蚴病是一种重要的食源性寄生人畜共患病；然而，关于中国湖南省蛇感染裂头蚴的流行情况的报道却很少。因此，我们于2018年调查了该地区野生蛇类中裂头蚴感染的流行情况，发现感染率惊人地达到了91.7%（344/375）。在99.1%的乌梢蛇、94.1%的王锦蛇和86.7%的黑眉锦蛇中发现了裂头蚴寄生虫。寄生虫呈现出多种分布情况：50%位于肌肉组织中，32.1%位于皮下组织中，17.9%位于体腔中。为了确定从野生蛇类中采集的裂头蚴的具体情况，对部分线粒体细胞色素c氧化酶亚基1（cox1）基因序列进行了扩增、测序和分析。所有检测的裂头蚴中，cox1的序列变异范围在0.0%至2.9%之间，共鉴定出21个可变位点（4.71%，21/446）。系统发育分析确定，从湖南省分离出的所有裂头蚴均为曼氏迭宫绦虫。这是湖南省蛇类感染曼氏迭宫绦虫的首次报道。应宣传裂头蚴病的风险和危害，并控制非法野生动物贸易。
