通过凝胶渗透色谱法测定新合成的含胍基二硫化物的聚（酰胺胺）的分子量。
Molecular weight determination of a newly synthesized guanidinylated disulfide-containing poly(amido amine) by gel permeation chromatography.
作者信息
Xing Haonan, Lu Mei, Xian Lei, Zhang Jinmin, Yang Tianzhi, Yang Li, Ding Pingtian
机构信息
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
Department of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, ME, USA.
出版信息
Asian J Pharm Sci. 2017 May;12(3):292-298. doi: 10.1016/j.ajps.2016.11.001. Epub 2016 Nov 18.
A cationic gene delivery vector, guanidinylated disulfide-containing poly(amido amine) (CAR-CBA), was synthesized by Michael addition reaction between N,N'-cystaminebisacrylamide (CBA) and guanidine hydrochloride (CAR). Gel permeation chromatography (GPC) was used to evaluate the molecular weight of synthesized CAR-CBA. Polyethyleneimine (PEI) with molecular weight of 25 kDa was adopted as a reference, and polyethylene glycols (PEG) with different molecular weights were used to establish a standard curve for determining the molecular weight of CAR-CBA. The effects of two critical factors, namely columns and eluents, on the molecular weight measurement of CAR-CBA were investigated to optimize the GPC quantitative method. The results showed that Ultrahydrogel columns (120, 250) and HAc-NaAc (0.5 M, pH 4.5) buffer solution were the optimal column and GPC eluent, respectively. The molecular weight of the synthesized CAR-CBA was analyzed by the optimized GPC method and determined to be 24.66 kDa.
通过N，N'-胱胺双丙烯酰胺（CBA）与盐酸胍（CAR）之间的迈克尔加成反应合成了一种阳离子基因递送载体——含胍基二硫键的聚（酰胺胺）（CAR-CBA）。采用凝胶渗透色谱法（GPC）评估合成的CAR-CBA的分子量。以分子量为25 kDa的聚乙烯亚胺（PEI）作为参比，并使用不同分子量的聚乙二醇（PEG）建立用于测定CAR-CBA分子量的标准曲线。研究了柱和洗脱剂这两个关键因素对CAR-CBA分子量测定的影响，以优化GPC定量方法。结果表明，Ultrahydrogel柱（120、250）和HAc-NaAc（0.5 M，pH 4.5）缓冲溶液分别是最佳的柱和GPC洗脱剂。通过优化的GPC方法分析合成的CAR-CBA的分子量，确定其为24.66 kDa。
Zotero 插件