Yamane Takumi, Konno Ryosuke, Iwatsuki Ken, Oishi Yuichi
Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan.
Amino Acids. 2020 Apr;52(4):505-510. doi: 10.1007/s00726-020-02831-6. Epub 2020 Mar 4.
Protein malnutrition is largely associated with a delay or failure of the healing process. However, the effect of dietary protein quality on wound healing is largely unknown. This study aimed to reveal the effect of dietary protein quality on wound healing and elucidate the regulatory mechanisms in a rat model of full-thickness cutaneous wounds. Rats were fed a normal diet for a week, and then they were divided into three groups that were fed the following diet for the experimental period: casein diet, gluten diet and gluten + lysine diet. The gluten diet significantly decreased body weight and wound healing compared with the casein diet, but this effect was reversed by supplementation with lysine. The numbers of leukocytes were significantly higher in the skin of the gluten group than those in the casein group. The wounded skin tissues of the gluten group showed lower amounts of collagen deposition compared with that in the casein group. Our results also showed that both matrix metalloproteinase (MMP) 2 activity and MMP14 mRNA levels were significantly increased in the skin of the gluten group, compared with the casein group. In summary, this study suggests low-quality protein diets have negative effects on wound healing via modulation of MMP2 activity in rats.
蛋白质营养不良在很大程度上与愈合过程的延迟或失败有关。然而，膳食蛋白质质量对伤口愈合的影响在很大程度上尚不清楚。本研究旨在揭示膳食蛋白质质量对伤口愈合的影响，并阐明全层皮肤伤口大鼠模型中的调节机制。大鼠先正常饮食一周，然后分为三组，在实验期间分别给予以下饮食：酪蛋白饮食、谷蛋白饮食和谷蛋白+赖氨酸饮食。与酪蛋白饮食相比，谷蛋白饮食显著降低了体重和伤口愈合，但补充赖氨酸可逆转这种影响。谷蛋白组皮肤中的白细胞数量显著高于酪蛋白组。与酪蛋白组相比，谷蛋白组受伤皮肤组织中的胶原蛋白沉积量较低。我们的结果还表明，与酪蛋白组相比，谷蛋白组皮肤中的基质金属蛋白酶（MMP）2活性和MMP14 mRNA水平均显著升高。总之，本研究表明低质量蛋白质饮食通过调节大鼠MMP2活性对伤口愈合产生负面影响。
