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氮杂甾醇对植物甾醇生物合成的抑制作用。

Inhibition of Phytosterol Biosynthesis by Azasterols.

作者信息

Darnet Sylvain, Martin Laetitia B B, Mercier Pierre, Bracher Franz, Geoffroy Philippe, Schaller Hubert

机构信息

CVACBA, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-750, Brazil.

Plant Isoprenoid Biology (PIB) team, Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, 67084 Strasbourg, France.

出版信息

Molecules. 2020 Mar 2;25(5):1111. doi: 10.3390/molecules25051111.

DOI:10.3390/molecules25051111
PMID:32131509
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7179204/
Abstract

Inhibitors of enzymes in essential cellular pathways are potent probes to decipher intricate physiological functions of biomolecules. The analysis of sterol profiles upon treatment with a series of azasterols reveals a specific in vivo inhibition of SMT2, a plant sterol-C-methyltransferase acting as a branch point between the campesterol and sitosterol biosynthetic segments in the pathway. Side chain azasteroids that modify sitosterol homeostasis help to refine its particular function in plant development.

摘要

关键细胞途径中酶的抑制剂是破译生物分子复杂生理功能的有力探针。用一系列氮杂甾醇处理后的甾醇谱分析揭示了对SMT2的特异性体内抑制，SMT2是一种植物甾醇-C-甲基转移酶，在该途径中作为菜油甾醇和谷甾醇生物合成段之间的分支点。修饰谷甾醇稳态的侧链氮杂甾体有助于明确其在植物发育中的特定功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/1578df648ba7/molecules-25-01111-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/b4f9f889f88d/molecules-25-01111-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/ece1077eaf51/molecules-25-01111-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/34d70ec300c7/molecules-25-01111-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/fcbc584f3adc/molecules-25-01111-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/50cc373a88eb/molecules-25-01111-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/63d3ebf924ec/molecules-25-01111-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/1578df648ba7/molecules-25-01111-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/b4f9f889f88d/molecules-25-01111-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/ece1077eaf51/molecules-25-01111-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/34d70ec300c7/molecules-25-01111-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/fcbc584f3adc/molecules-25-01111-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/50cc373a88eb/molecules-25-01111-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/63d3ebf924ec/molecules-25-01111-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a86a/7179204/1578df648ba7/molecules-25-01111-g007.jpg

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