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微小RNA-18a抑制剂通过上调PTEN表达抑制白血病细胞增殖。

miR-18a Inhibitor Suppresses Leukemia Cell Proliferation by Upregulation of PTEN Expression.

作者信息

Zhang Liping, Kong Lingxia, Yang Yuzhi

机构信息

Department of Hematology, Dezhou People's Hospital, Dezhou, Shandong, China (mainland).

Department of Respiratory Medicine, Dezhou People's Hospital, Dezhou, Shandong, China (mainland).

出版信息

Med Sci Monit. 2020 Mar 8;26:e921288. doi: 10.12659/MSM.921288.

DOI:10.12659/MSM.921288
PMID:32146479
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7081926/
Abstract

BACKGROUND Leukemia is common in aging adults and has very high mortality worldwide. The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1 cell proliferative potential was measured by use of the Cell Counting Kit-8 assay. Apoptotic changes were analyzed by electron microscopy, and evaluation of PI3K, AKT, mTOR, and PTEN expression was assessed by RT-qPCR assay. RESULTS Transfection of miR-18a inhibitor significantly (P<0.05) suppressed the proliferative potential of WEHI-3 and THP-1 cells. The WEHI-3 cells showed the presence of characteristic apoptotic bodies on transfection with miR-18a inhibitor at 48 h. Flow cytometry showed that miR-18a inhibitor transfection significantly (P<0.05) increased the WEHI-3 cell percentage in G1 phase. The transfection of miR-18a inhibitor significantly (P<0.05) promoted apoptosis in WEHI-3 cells. In WEHI-3 cells, miR-18a inhibitor transfection markedly suppressed the expression of PI3K, AKT, and mTOR mRNA. The expression of PTEN mRNA was significantly (P<0.05) upregulated by miR-18a inhibitor transfection in WEHI-3 cells. CONCLUSIONS The present study investigated the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP1 leukemia cells. The study demonstrated that miR-18a inhibitor suppressed the proliferative potential of WEHI-3 and THP1 cells and activated apoptotic process through upregulation of PTEN mRNA expression. Therefore, miR-18a inhibitor can be of therapeutic importance for the treatment of leukemia.

摘要

背景

白血病在老年人群中较为常见，在全球范围内死亡率极高。本研究旨在探讨miR-18a抑制剂对WEHI-3和THP-1白血病细胞的治疗效果。

材料与方法

使用细胞计数试剂盒-8检测法测定转染miR-18a抑制剂的WEHI-3和THP-1细胞增殖潜能的变化。通过电子显微镜分析凋亡变化，并采用RT-qPCR检测法评估PI3K、AKT、mTOR和PTEN的表达。

结果

转染miR-18a抑制剂显著（P<0.05）抑制了WEHI-3和THP-1细胞的增殖潜能。在转染miR-18a抑制剂48小时后，WEHI-3细胞出现典型的凋亡小体。流式细胞术显示，转染miR-18a抑制剂显著（P<0.05）增加了G1期WEHI-3细胞的比例。转染miR-18a抑制剂显著（P<0.05）促进了WEHI-3细胞的凋亡。在WEHI-3细胞中，转染miR-18a抑制剂显著抑制了PI3K、AKT和mTOR mRNA的表达。在WEHI-3细胞中，转染miR-18a抑制剂显著（P<0.05）上调了PTEN mRNA的表达。

结论

本研究探讨了miR-18a抑制剂对WEHI-3和THP1白血病细胞的治疗效果。研究表明，miR-18a抑制剂通过上调PTEN mRNA表达抑制了WEHI-3和THP1细胞的增殖潜能并激活了凋亡过程。因此，miR-18a抑制剂在白血病治疗中可能具有重要的治疗意义。

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