更格卢鼠（Ordii更格卢鼠）3.3千碱基重复DNA元件的基因组结构与序列变异

Genomic structure and sequence variation of a 3.3-kb repeat DNA element of the kangaroo rat, Dipodomys ordii.

作者信息

Kursar T A

机构信息

Department of Biology, University of Utah, Salt Lake City 84112.

出版信息

Gene. 1988 Oct 30;70(2):263-70. doi: 10.1016/0378-1119(88)90198-9.

DOI:10.1016/0378-1119(88)90198-9

PMID:3215518

Abstract

The genomic structure and sequence variation of a 3.3-kb repeat DNA element, representing 5% of the genome of the kangaroo rat Dipodomys ordii, has been investigated. Most of the repeats are arranged in tandem arrays of 50 kb or more. Thirteen randomly selected genomic clones have been mapped with twelve restriction enzymes. The frequency of sequence divergence in the genomic clones is 0.5%. The clone maps and the genomic structure studies have permitted the characterization of a number of variant members of the 3.3-kb repeat family. The genomic organization of the repeat resembles that for repeated DNAs found in large tandem arrays or satellites.

摘要

对一种3.3kb重复DNA元件的基因组结构和序列变异进行了研究，该元件占更格卢鼠（Dipodomys ordii）基因组的5%。大多数重复序列以50kb或更长的串联阵列形式排列。用12种限制性内切酶对13个随机选择的基因组克隆进行了图谱绘制。基因组克隆中序列分歧的频率为0.5%。克隆图谱和基因组结构研究使得能够对3.3kb重复家族的一些变异成员进行表征。该重复序列的基因组组织类似于在大型串联阵列或卫星中发现的重复DNA的基因组组织。