Department of Life Sciences, Incheon National University, Incheon 22012, Korea.
Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon 22012, Korea.
Cells. 2020 Mar 6;9(3):639. doi: 10.3390/cells9030639.
Intercellular communication between vascular smooth muscle cells (VSMCs) and endothelial cells (ECs) is essential for the maintenance of vascular homeostasis. The presence of exosomes, a recently discovered player in vascular cell communication, has been associated with vascular disease progression. However, the detailed mechanism of how the signal mediated by exosomes affects the function of vascular cells during vascular pathogenesis is yet to be further understood. In this study, we investigated the expression of exosomal microRNAs (miRNAs) secreted by VSMCs and their functional relevance to ECs in pathogenesis, including their role in processes such as platelet-derived growth factor (PDGF) stimulation. We observed that PDGF stimulation contributes to a change in exosomal miRNA release from VSMCs; specifically, miR-1246, miR-182, and miR-486 were deficient in exosomes derived from PDGF-stimulated VSMCs. The reduced miRNA expression in these exosomes is associated with an increase in EC migration. These findings increase our understanding of exosome-mediated crosstalk between vascular cells under a pathological condition.
血管平滑肌细胞(VSMCs)和内皮细胞(ECs)之间的细胞间通讯对于维持血管内环境稳态至关重要。外泌体是一种最近发现的血管细胞通讯中的参与者，它与血管疾病的进展有关。然而，外泌体介导的信号如何影响血管细胞在血管发病机制中的功能的详细机制仍有待进一步理解。在这项研究中，我们研究了 VSMCs 分泌的外泌体 microRNAs (miRNAs)的表达及其在发病机制中对 ECs 的功能相关性，包括它们在血小板衍生生长因子(PDGF)刺激等过程中的作用。我们观察到，PDGF 刺激导致 VSMCs 释放的外泌体 miRNA 发生变化；具体而言，miR-1246、miR-182 和 miR-486 在 PDGF 刺激的 VSMCs 衍生的外泌体中缺失。这些外泌体中 miRNA 表达的减少与 EC 迁移的增加有关。这些发现增加了我们对病理条件下血管细胞之间外泌体介导的串扰的理解。
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