Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, 08826, Republic of Korea.
Department of Brain and Cognitive Engineering, Korea University, Seoul, 02841, Republic of Korea.
Appl Biochem Biotechnol. 2020 Aug;191(4):1545-1561. doi: 10.1007/s12010-020-03271-4. Epub 2020 Mar 11.
The role of actin fibers in cellular responses to external electric pulses is not clear yet. In this study, we utilized the blocker of actin polymerization, cytochalasin D (cytoD), and investigated its effects on the electropore generation. Eight 100 μs electric pulses of sub-kilovolt per centimeter voltage with 100 ms intervals were applied to adhered cells in vitro, and the membrane permeability was quantified using membrane-impermeable propidium iodide (PI) dye. With cytoD application, the transfer of PI dye decreased significantly in all the applied voltages. At the same time, the roughness of cells increased, the membrane stiffness decreased, and the transmembrane resting potential decreased. Our result supports that actin fibers have clear effects on electroporation through modulating membrane properties including transmembrane resting potential.
肌动蛋白纤维在细胞对外界电脉冲的反应中的作用尚不清楚。在这项研究中，我们利用肌动蛋白聚合抑制剂细胞松弛素 D（cytoD），并研究其对电穿孔的影响。将 8 个 100μs、亚千伏每厘米电压的电脉冲以 100ms 的间隔施加到体外贴壁细胞上，并用膜不可渗透的碘化丙啶（PI）染料定量测量细胞膜通透性。应用 cytoD 后，所有施加电压下的 PI 染料转移均显著减少。同时，细胞粗糙度增加，膜硬度降低，跨膜静息电位降低。我们的结果支持肌动蛋白纤维通过调节包括跨膜静息电位在内的膜性质对电穿孔有明显影响。
