Hirano M, Goldman J E
Department of Pathology, Albert Einstein College of Medicine, Bronx, New York.
J Neurosci Res. 1988 Oct-Dec;21(2-4):155-67. doi: 10.1002/jnr.490210208.
We have examined glial cell lineages during rat spinal cord development by using a variety of antibodies that react with immature and mature glia. Radial glia in embryonic cord bound 1) A2B5, an antibody that reacts with a glial precursor cell population in optic nerve; 2) AbR24, which is directed against GD3 ganglioside and binds to immature neuroectodermal cells and to developing oligodendrocytes in forebrain and cerebellum; and 3) an antibody to the intermediate filament, vimentin. With time, two different populations emerged, both of which seemed to be derivatives of radial cells. One cell type expressed the astrocyte intermediate filament, GFAP, in addition to vimentin. GFAP-containing cells eventually took on the forms of astrocytes in gray and white matter. The other type expressed carbonic anhydrase, an enzyme characteristic of oligodendrocytes and enriched in myelin. Carbonic anhydrase-positive cells eventually developed into small cells with oligodendrocyte morphology. Our observations suggest a common lineage for astrocytes and oligodendrocytes from radial cells during spinal cord gliogenesis.
我们通过使用多种与未成熟和成熟神经胶质细胞发生反应的抗体，研究了大鼠脊髓发育过程中的神经胶质细胞谱系。胚胎脊髓中的放射状胶质细胞结合了：1）A2B5，一种与视神经中的神经胶质前体细胞群体发生反应的抗体；2）AbR24，它针对GD3神经节苷脂，与未成熟的神经外胚层细胞以及前脑和小脑中正在发育的少突胶质细胞结合；3）一种针对中间丝波形蛋白的抗体。随着时间的推移，出现了两种不同的细胞群体，它们似乎都是放射状细胞的衍生物。一种细胞类型除了波形蛋白外，还表达星形胶质细胞中间丝GFAP。含GFAP的细胞最终形成了灰质和白质中的星形胶质细胞形态。另一种类型表达碳酸酐酶，这是少突胶质细胞的一种特征性酶，在髓鞘中含量丰富。碳酸酐酶阳性细胞最终发育成具有少突胶质细胞形态的小细胞。我们的观察结果表明，在脊髓神经胶质发生过程中，星形胶质细胞和少突胶质细胞来自放射状细胞的共同谱系。
