将光致变色受体对磷酸化阴离子的识别能力进行转换。

Switching the recognition ability of a photoswitchable receptor towards phosphorylated anions.

机构信息

Department of Chemical Sciences, Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur, Nadia, West Bengal 741246, India.

Institute of Organic Chemistry, University of Duisburg-Essen, Universitatsstraße 745141, Essen, Germany.

出版信息

Chem Commun (Camb). 2020 Apr 14;56(30):4172-4175. doi: 10.1039/d0cc00926a.

DOI:10.1039/d0cc00926a

PMID:32167107

Abstract

An azobenzene based photoswitchable macrocyclic receptor displays different binding affinities in its E and Z forms towards various phosphorylated coenzymes under physiological conditions with remarkable selectivity for ATP in the E-form and selectivity towards GTP in the photoisomerized Z-form. Linear discriminant analysis clearly separated the analytes using the E-form. An application of this method enabled monitoring the progress of enzymatic phosphorylation using a tyrosine kinase enzyme.

摘要

一种基于偶氮苯的光致变色大环受体在生理条件下以 E 和 Z 形式对各种磷酸化辅酶表现出不同的结合亲和力，在 E 形式下对 ATP 具有显著的选择性，在光致异构化的 Z 形式下对 GTP 具有选择性。线性判别分析清楚地使用 E 形式分离了分析物。该方法的应用可用于监测酪氨酸激酶酶的酶促磷酸化过程。