College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, PR China.
College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, PR China; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China.
Ecotoxicol Environ Saf. 2020 May;194:110435. doi: 10.1016/j.ecoenv.2020.110435. Epub 2020 Mar 10.
Soil salinization is one of most crucial environmental problems around the world and negatively affects plant growth and production. Carex rigescens is a turfgrass with favorable stress tolerance and great application prospect in salinity soil remediation and utilization; however, the molecular mechanisms behind its salt stress response are unknown. We performed a time-course transcriptome analysis between salt tolerant 'Huanghua' (HH) and salt sensitive 'Beijing' (BJ) genotypes. Physiological changes within 24 h were observed, with the HH genotype exhibiting increased salt tolerance compared to BJ. 5764 and 10752 differentially expressed genes were approved by transcriptome in BJ and HH genotype, respectively, and dynamic analysis showed a discrepant profile between two genotypes. In the BJ genotype, genes related to carbohydrate metabolism and stress response were more active and ABA signal transduction pathway might play a more important role in salt stress tolerance than in HH genotype. In the HH genotype, unique increases in the regulatory network of transcription factors, hormone signal transduction, and oxidation-reduction processes were observed. Moreover, trehalose and pectin biosynthesis and chitin catabolic related genes were specifically involved in the HH genotype, which may have contributed to salt tolerance. Moreover, some candidate genes like mannan endo-1,4-beta-mannosidase and EG45-like domain-containing protein are highlighted for future research about salt stress resistance in C. rigescens and other plant species. Our study revealed unique salt adaptation and resistance characteristics of two C. rigescens genotypes and these findings could help to enrich the currently available knowledge and clarify the detailed salt stress regulatory mechanisms in C. rigescens and other plants.
土壤盐渍化是全球最主要的环境问题之一，它会对植物的生长和产量产生负面影响。羊胡子草是一种具有良好抗逆性的草坪草，在盐渍土修复和利用方面具有广阔的应用前景；然而，其对盐胁迫响应的分子机制尚不清楚。我们对耐盐‘黄花’（HH）和盐敏感‘北京’（BJ）基因型之间进行了时间进程转录组分析。观察到 24 小时内的生理变化，HH 基因型比 BJ 基因型表现出更高的耐盐性。在 BJ 和 HH 基因型中分别有 5764 个和 10752 个差异表达基因通过转录组得到证实，动态分析显示两种基因型之间的差异较大。在 BJ 基因型中，与碳水化合物代谢和应激反应相关的基因更为活跃，ABA 信号转导途径在耐盐性方面可能比 HH 基因型更为重要。在 HH 基因型中，观察到转录因子调控网络、激素信号转导和氧化还原过程的独特增加。此外，海藻糖和果胶生物合成以及几丁质分解相关基因特异性参与 HH 基因型，这可能有助于其耐盐性。此外，甘露聚糖内切 1,4-β-甘露糖苷酶和 EG45 样结构域蛋白等一些候选基因被突出强调，以便未来对羊胡子草和其他植物物种的耐盐性进行研究。本研究揭示了两种羊胡子草基因型独特的耐盐适应和抗性特征，这些发现有助于丰富目前可用的知识，并阐明羊胡子草和其他植物中详细的盐胁迫调控机制。
