沙特阿拉伯用于识别的扫描电子显微镜和分子方法。
SEM and molecular approaches to identify in Saudi Arabia.
作者信息
Soliman O, Montaser M M, Ashour A A, Soliman M I, Nigm A H
机构信息
1Zoology Department, Faculty of Science, Ain-Shams University, Cairo, Egypt.
2Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
出版信息
J Parasit Dis. 2020 Mar;44(1):239-247. doi: 10.1007/s12639-019-01187-3. Epub 2019 Dec 23.
In the present study, (Paramphistomidae), a parasite of rumina of sheep and cows, was collected from Taif region, KSA, to investigate its identity and the prevalence of infection in livestock. Specific identification was mainly achieved by ITS molecular technique and surface ultrastructure was studied by SEM. Adult is described for the first time from Saudi Arabia. The infection rate is 1% in sheep and 10% in cows. Results indicated that this species isolate was genetically identical with other isolates where matching reach 100%. The primary sequence of the rDNA ITS2+ region of the fluke produces amplicon of 647 bp. SEM results show that no observed papillae around its genital opening. Besides, it has very few scattered small papillae around its oral region with few randomly distributed acetabular papillae. These findings were compared with the same corresponding species in other previous works of literatures.
在本研究中，从沙特阿拉伯王国塔伊夫地区采集了羊和牛反刍动物的寄生虫（双腔科），以调查其身份及家畜中的感染率。主要通过ITS分子技术进行特异性鉴定，并通过扫描电子显微镜研究表面超微结构。首次从沙特阿拉伯描述了该成虫。绵羊的感染率为1%，牛的感染率为10%。结果表明，该物种分离株在基因上与其他分离株相同，匹配率达到100%。吸虫的rDNA ITS2+区域的一级序列产生647 bp的扩增子。扫描电子显微镜结果显示，在其生殖孔周围未观察到乳头。此外，在其口部区域周围有很少分散的小乳头，并有少量随机分布的腹吸盘乳头。将这些发现与以往其他文献中相同相应物种的情况进行了比较。
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