Zhang Pan, Shao Hudie, You Chunping, Feng Yan, Xie Zhenwen
College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering , Guangzhou, Guangdong, 510225, China.
College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering , Guangzhou, Guangdong, 510225, China ; College of Agriculture, Yangtze University , Jingzhou, Hubei, 434025, China.
J Nematol. 2020;52:1-8. doi: 10.21307/jofnem-2020-004.
China is one of the largest producers of mulberry in the world. With the development of the sericulture industry, several pests and diseases have occurred in rapid succession, chief among which is the root-knot nematode disease affecting mulberry. According to the China cocoon and silk exchange, cocoon prices have doubled since the beginning of 2009 and rose to 92,700 yuan ($135,770) per tonne in mid-April 2010. According to customs statistics, in the first eight months of 2011, China's silk merchandise exports amounted to 2.39 billion yuan. In this study, sequencing of the rDNA-ITS and D2-D3 region of the 28S rRNA gene was combined with root-knot nematode morphological characteristics to identify the root-knot nematode infecting mulberry in the Guangdong, Guangxi, and Hunan provinces of China. This resulted in the identification of as the causal species of root-knot nematode infections in these regions. Importantly, the morphological data agreed completely with our molecular phenotyping efforts, indicating that rDNA sequencing could provide a more clear-cut and less labor-intensive means of characterizing root-knot nematode infections in the future. The differences between this study and the previous studies were discussed, as well as the damage degree, host species and influence scope of . China is one of the largest producers of mulberry in the world. With the development of the sericulture industry, several pests and diseases have occurred in rapid succession, chief among which is the root-knot nematode disease affecting mulberry. According to the China cocoon and silk exchange, cocoon prices have doubled since the beginning of 2009 and rose to 92,700 yuan ($135,770) per tonne in mid-April 2010. According to customs statistics, in the first eight months of 2011, China’s silk merchandise exports amounted to 2.39 billion yuan. In this study, sequencing of the rDNA-ITS and D2-D3 region of the 28S rRNA gene was combined with root-knot nematode morphological characteristics to identify the root-knot nematode infecting mulberry in the Guangdong, Guangxi, and Hunan provinces of China. This resulted in the identification of as the causal species of root-knot nematode infections in these regions. Importantly, the morphological data agreed completely with our molecular phenotyping efforts, indicating that rDNA sequencing could provide a more clear-cut and less labor-intensive means of characterizing root-knot nematode infections in the future. The differences between this study and the previous studies were discussed, as well as the damage degree, host species and influence scope of .
中国是世界上最大的桑树生产国之一。随着蚕桑产业的发展，病虫害接连出现，其中主要是影响桑树的根结线虫病。据中国茧丝绸交易市场数据，自2009年初以来茧价翻了一番，2010年4月中旬涨至每吨92,700元（135,770美元）。据海关统计，2011年前八个月，中国丝绸商品出口额达23.9亿元。在本研究中，将28S rRNA基因的rDNA-ITS和D2-D3区域测序与根结线虫的形态特征相结合，以鉴定在中国广东、广西和湖南省感染桑树的根结线虫。这使得鉴定出 为这些地区根结线虫感染的致病物种。重要的是，形态学数据与我们的分子表型分析结果完全一致，表明rDNA测序在未来可提供一种更明确且省力的方法来鉴定根结线虫感染。讨论了本研究与先前研究的差异，以及 的损害程度、寄主物种和影响范围。中国是世界上最大的桑树生产国之一。随着蚕桑产业的发展，病虫害接连出现，其中主要是影响桑树的根结线虫病。据中国茧丝绸交易市场数据，自2009年初以来茧价翻了一番，2010年4月中旬涨至每吨92,700元（135,770美元）。据海关统计，2011年前八个月，中国丝绸商品出口额达23.9亿元。在本研究中，将28S rRNA基因的rDNA-ITS和D2-D3区域测序与根结线虫的形态特征相结合，以鉴定在中国广东、广西和湖南省感染桑树的根结线虫。这使得鉴定出 为这些地区根结线虫感染的致病物种。重要的是，形态学数据与我们的分子表型分析结果完全一致，表明rDNA测序在未来可提供一种更明确且省力的方法来鉴定根结线虫感染。讨论了本研究与先前研究的差异，以及 的损害程度、寄主物种和影响范围。
