Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Department of Korean Medicine, Graduate School of Wonkwang University, Iksan 54538, Republic of Korea.
J Tradit Chin Med. 2019 Jun;39(3):307-314.
OBJECTIVE: To investigate the effects of acupuncture with dexamethasone (A. Dex) on allergic rhinitis (AR) by injecting dexamethasone into the Zusanli (ST 36) acupoint. METHODS: Thirty 6-week-old female BALB/c mice were sensitized on days 1, 5, and 14 by intraperitoneal injection of 100 µg of ovalbumin (OVA). The mice were then randomly divided into six groups (n = 5 in each group). Five groups were sensitized intranasally with 2 μL of 1.5 mg of OVA for 10 consecutive days, while one group was sensitized intranasally with PBS in a similar manner as a negative control group. One hour before each administration of intranasal OVA, two groups were orally administered either a control vehicle (distilled water; AR control group) or 200 μg/kg Dex (O. Dex group), while three groups received A. Dex at Zusanli (ST 36) with Dex concentrations of 2, 20, and 200 μg/kg for each group, respectively. AR symptoms were evaluated by measuring the rubbing score, which comprised the number of nose, ear, and eye rubs that occurred in the initial 10 min after OVA intranasal provocation on the 10th day. We isolated spleen, serum, and nasal mucosal tissue after measuring the rubbing score. Spleen weight was measured using an electronic microbalance. The levels of IgE, thymic stromal lymphopoietin, tumor necro- sis factor-α, intercellular adhesion molecule-1, and macrophage-inflammatory protein-2 in serum or nasal mucosal tissue were measured using enzyme-linked immunosorbent assays. The serum histamine levels of OVA-sensitized AR mice were measured using O-phthaldialdehyde spectrofluorometry. Western blot analysis was performed on nasal mucosal tissue extracts. RESULTS: A. Dex significantly reduced the rubbing score, spleen weight, serum IgE, and serum histamine in OVA-sensitized mice. A. Dex significantly decreased the serum levels of inflammatory cytokines (thymic stromal lymphopoietin and tumor ne- crosis factor-α) in OVA-sensitized mice. A. Dex sig-nificantly reduced the nasal mucosal levels of inflammatory markers (intercellular adhesion molecule-1andmacrophage-inflammatory protein-2) inAR mice. A. Dex effectively attenuated the expression of caspase-1 and receptorinteractingprotein-2 in nasal mucosal tissue.
目的：通过在足三里（ST36）穴位注射地塞米松（A. Dex）来研究针刺加地塞米松对过敏性鼻炎（AR）的影响。
方法：将 30 只 6 周龄雌性 BALB/c 小鼠分别于第 1、5 和 14 天经腹腔注射 100μg 卵清蛋白（OVA）进行致敏。然后，将小鼠随机分为六组（每组 5 只）。五组通过鼻腔内给予 2μL 1.5mg OVA 连续 10 天进行致敏，一组以类似方式鼻腔内给予 PBS 作为阴性对照组。在每次鼻腔内给予 OVA 前 1 小时，两组分别口服对照液（蒸馏水；AR 对照组）或 200μg/kg Dex（O.Dex 组），三组分别在足三里（ST36）穴位接受 A.Dex，Dex 浓度分别为 2、20 和 200μg/kg。在第 10 天，在 OVA 鼻腔激发后最初 10 分钟内测量鼻、耳和眼揉鼻次数，评估 AR 症状。测量揉鼻评分后，分离脾脏、血清和鼻黏膜组织。使用电子微量天平测量脾脏重量。使用酶联免疫吸附试验测量血清或鼻黏膜组织中 IgE、胸腺基质淋巴细胞生成素、肿瘤坏死因子-α、细胞间黏附分子-1 和巨噬细胞炎症蛋白-2 的水平。使用邻苯二甲醛荧光法测量 OVA 致敏 AR 小鼠的血清组胺水平。对鼻黏膜组织提取物进行 Western blot 分析。
结果：A.Dex 可显著降低 OVA 致敏小鼠的揉鼻评分、脾脏重量、血清 IgE 和血清组胺。A.Dex 可显著降低 OVA 致敏小鼠的血清炎症细胞因子（胸腺基质淋巴细胞生成素和肿瘤坏死因子-α）水平。A.Dex 可显著降低 AR 小鼠鼻黏膜组织中炎症标志物（细胞间黏附分子-1 和巨噬细胞炎症蛋白-2）水平。A.Dex 可有效抑制鼻黏膜组织中半胱天冬酶-1 和受体相互作用蛋白-2 的表达。
Arch Dermatol Res. 2016-3
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