Luo Dan, Qu Lina, Zhong Ming, Li Xinmei, Wang Han, Miao Jiahui, Liu Xuanming, Zhao Xiaoying
College of Biology, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University , Changsha, China.
Shenzhen Institute, Hunan University , Shenzhen, China.
Biosci Biotechnol Biochem. 2020 Jul;84(7):1384-1393. doi: 10.1080/09168451.2020.1740971. Epub 2020 Mar 18.
Seed germination is regulated by light. Phytochromes (Phys) act as red and far-red light photoreceptors to mediate seed germination. However, the mechanism of this process is not well understood. In this study, we found that the mutants () and showed higher seed germination percentage than wild type when PhyB was inactivated by far-red light. In wild type, and expression were downregulated after seed imbibition, repressed by PhyB, and upregulated by Phytochrome-interacting factor 1 (PIF1), a key negative regulator of seed germination. Red light irradiation and the mutation caused increased expression of (), a gibberellin (GA) biosynthetic gene. We also found that VOZ2 is bound directly to the promoter of and . Our findings suggest that VOZs play a negative role in PhyB-mediated seed germination, possibly by directly regulating expression.
种子萌发受光调控。光敏色素（Phys）作为红光和远红光光感受器介导种子萌发。然而，这一过程的机制尚未完全清楚。在本研究中，我们发现当phyB被远红光失活时，突变体（）和表现出比野生型更高的种子萌发率。在野生型中，种子吸胀后和的表达下调，受phyB抑制，并被种子萌发的关键负调节因子光敏色素相互作用因子1（PIF1）上调。红光照射和突变导致赤霉素（GA）生物合成基因（）的表达增加。我们还发现VOZ2直接与和的启动子结合。我们的研究结果表明，VOZs可能通过直接调节的表达，在phyB介导的种子萌发中起负作用。
