人体呼吸测试中一氧化氮的紫外区域光腔衰荡光谱。

Cavity ringdown spectroscopy of nitric oxide in the ultraviolet region for human breath test.

机构信息

Institute of Biomedical Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin, People's Republic of China. Department of Physics and Astronomy, Mississippi State University, Starkville, Mississippi, United States of America.

出版信息

J Breath Res. 2020 May 1;14(3):037101. doi: 10.1088/1752-7163/ab8184.

DOI:10.1088/1752-7163/ab8184

PMID:32191922

Abstract

We report the spectrum of nitric oxide (NO) in the ultraviolet (UV) (225.4-227.0 nm) region based on cavity ringdown spectroscopy (CRDS). A cavity ringdown system, which consisted of a tunable UV laser source and a vacuum-pumped ringdown cavity, was constructed to measure NO at room temperature and atmospheric or reduced pressure. The measured spectra were validated using LIFBase simulations. The absorption cross-section of NO at the strongest absorption peak at 226.255 nm was measured to be 7.64 × 10 cm molecule. Using the measured mirror reflectivity of 99.55% at 226.255 nm, the detection limit of NO was determined to be 7.4 ppb (parts per billion) based on the standard 3-σ criteria. The stability and reproducibility of this CRDS system were also tested. Furthermore, exhaled gas samples from 203 human subjects (105 healthy people and 98 lung cancer patients) were measured using the system. Results demonstrated that the cavity ringdown spectroscopy in the deep-UV region has potential for breath NO test.

摘要

我们基于腔衰荡光谱（CRDS）报告了一氧化氮（NO）在紫外（UV）（225.4-227.0nm）区域的光谱。构建了一个腔衰荡系统，该系统由可调谐 UV 激光源和真空抽运的衰荡腔组成，用于在室温以及大气或减压条件下测量 NO。使用 LIFBase 模拟验证了测量的光谱。在 226.255nm 的最强吸收峰处测量到的 NO 的吸收截面为 7.64×10cm 分子。使用在 226.255nm 处的 99.55%的测量镜反射率，根据标准 3-σ 准则，将 NO 的检测限确定为 7.4ppb（十亿分之一）。还测试了该 CRDS 系统的稳定性和重现性。此外，使用该系统测量了来自 203 个人体（105 名健康人和 98 名肺癌患者）的呼气样本。结果表明，深紫外区域的腔衰荡光谱在呼吸 NO 测试方面具有潜力。