3D 同轴生物打印血管

3D Coaxial Bioprinting of Vasculature.

机构信息

Engineering Science and Mechanics Department, Penn State University, University Park, PA, USA.

The Huck Institutes of the Life Sciences, Penn State University, University Park, PA, USA.

出版信息

Methods Mol Biol. 2020;2140:171-181. doi: 10.1007/978-1-0716-0520-2_11.

DOI:10.1007/978-1-0716-0520-2_11

PMID:32207112

Abstract

Development of a suitable vascular network for an efficient mass exchange is crucial to generate three-dimensional (3D) viable and functional thick construct in tissue engineering. Different technologies have been reported for the fabrication of vasculature conduits, such as decellularized tissues and biomaterial-based blood vessels. Recently, bioprinting has also been considered as a promising method in vascular tissue engineering. In this work, human umbilical vein smooth muscle cells (HUVSMCs) were encapsulated in sodium alginate and printed in the form of vasculature conduits using a coaxial nozzle deposition system. Protocols for cell encapsulation and 3D bioprinting are presented. Investigations including dehydration, swelling, degradation characteristics, and patency, permeability, and mechanical properties were also performed and presented to the reader. In addition, in vitro studies such as cell viability and evaluation of extra cellular matrix deposition were performed.

摘要

为了在组织工程中生成三维（3D）可行且功能齐全的厚构建体，开发适合的血管网络以实现有效的物质交换至关重要。已经有报道称，不同的技术可用于制造脉管结构，例如脱细胞组织和基于生物材料的血管。最近，生物打印也被认为是血管组织工程中的一种有前途的方法。在这项工作中，人脐静脉平滑肌细胞（HUVSMCs）被包裹在海藻酸钠中，并使用同轴喷嘴沉积系统以脉管结构的形式进行打印。本文提出了细胞包封和 3D 生物打印的方案。还进行了包括脱水、溶胀、降解特性以及通畅性、渗透性和机械性能的研究，并呈现给读者。此外，还进行了体外研究，例如细胞活力和细胞外基质沉积的评估。