异位骨化爆炸和截肢大鼠模型中血清睾酮和皮质酮水平的特征。
Characterization of serum levels of testosterone and corticosterone in a blast and amputation rat model of heterotopic ossification.
机构信息
Naval Medical Research Center, Regenerative Medicine, Silver Spring, MD, USA; Uniformed Services University of the Health Sciences, Department of Surgery, Bethesda, MD, USA.
Uniformed Services University of the Health Sciences, Department of Surgery, Bethesda, MD, USA.
出版信息
Mol Cell Endocrinol. 2020 Jun 1;509:110799. doi: 10.1016/j.mce.2020.110799. Epub 2020 Mar 21.
INTRODUCTION
Endocrine dysregulation's role in heterotopic ossification (HO) remains unexplored. We sought to examine corticosterone and testosterone in established rat models of ectopic bone formation, and correlate to HO formation with CT analysis.
METHODS
Fifteen rats were placed into three groups of traumatic injury patterns: Blast and injury (120 kPa blast, femoral fracture and quadriceps crush), injury only, and blast only. Serum corticosterone and testosterone levels were drawn until post-operative day 40. HO was analyzed using CT.
RESULTS
Corticosterone levels peaked in the blast and injury group in the shortest time post injury, followed by injury only and blast only groups. Testosterone levels reached nadir in similar fashion. Volume of HO was highest in the blast and injury group, followed by the injury only group.
CONCLUSION
Corticosterone and testosterone's contribution to HO formation requires further characterization, but this study suggests that high peaks in corticosterone and a low nadir in testosterone are associated with higher volumes of HO.
简介
内分泌失调在异位骨化（HO）中的作用尚不清楚。我们试图在异位骨形成的已建立的大鼠模型中检查皮质酮和睾酮，并通过 CT 分析将其与 HO 形成相关联。
方法
将 15 只大鼠分为三组创伤性损伤模式：爆炸和损伤（120kPa 爆炸，股骨干骨折和股四头肌挤压伤），仅损伤和仅爆炸。在术后第 40 天之前抽取血清皮质酮和睾酮水平。使用 CT 分析 HO。
结果
皮质酮水平在受伤后最短时间内的爆炸和损伤组中达到峰值，其次是仅损伤组和仅爆炸组。睾酮水平以相似的方式达到最低点。HO 的体积在爆炸和损伤组中最高，其次是仅损伤组。
结论
皮质酮和睾酮对 HO 形成的贡献需要进一步的特征描述，但本研究表明，皮质酮的高峰和睾酮的低谷与 HO 体积较高有关。
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