核糖体突变与RNA聚合酶之间的相互作用：一对strA和rif突变体单独时对温度不敏感，但组合时对温度敏感。
Interaction between mutations of ribosomes and RNA polymerase: a pair of strA and rif mutants individually temperature-insensitive but temperature-sensitive in combination.
作者信息
Chakrabarti S L, Gorini L
出版信息
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1157-61. doi: 10.1073/pnas.74.3.1157.
Abstract
A temperature-sensitive lethal mutant of Escherichia coli has been constructed by combining two temperature-insensitive mutations: a rif180 mutation that modifies RNA polymerase (RNA nucleotidyltransferase; nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) and a strA24 mutation that modifies the ribosomal protein S12. The temperature sensitivity is a property of the combination of these two particular alleles; replacement of either of the alleles relieves the temperature sensitivity. An isogenic strain containing a different strA mutation (i.e., rif180 strA11) is not temperature sensitive. Evidently ribosomes modified by the particular strA24 polymerase altered by the rif180 mutation, which suggests that in vivo there may exist some interaction between structures of ribosomes and the RNA polymerase.
摘要
通过组合两个温度不敏感突变构建了大肠杆菌的温度敏感致死突变体
一个修饰RNA聚合酶（RNA核苷酸转移酶；核苷三磷酸：RNA核苷酸转移酶，EC 2.7.7.6）的rif180突变和一个修饰核糖体蛋白S12的strA24突变。温度敏感性是这两个特定等位基因组合的特性；替换其中任何一个等位基因都可缓解温度敏感性。含有不同strA突变（即rif180 strA11）的同基因菌株对温度不敏感。显然，由rif180突变改变的特定strA24聚合酶修饰的核糖体，这表明在体内核糖体结构与RNA聚合酶之间可能存在某种相互作用。
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