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转录组分析揭示鸭坦布苏病毒感染大脑中的神经免疫相互作用。

Transcriptome Analysis Reveals the Neuro-Immune Interactions in Duck Tembusu Virus-Infected Brain.

机构信息

Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture, Guangdong Open Laboratory of Veterinary Public Health, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China.

出版信息

Int J Mol Sci. 2020 Mar 31;21(7):2402. doi: 10.3390/ijms21072402.

DOI:10.3390/ijms21072402
PMID:32244328
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7177238/
Abstract

The duck Tembusu virus (DTMUV) is a mosquito-borne flavivirus. It causes severe symptoms of egg-drop, as well as neurological symptoms and brain damage in ducks. However, the specific molecular mechanisms of DTMUV-induced neurovirulence and host responses in the brain remain obscure. To better understand the host-pathogen and neuro-immune interactions of DTMUV infection, we conducted high-throughput RNA-sequencing to reveal the transcriptome profiles of DTMUV-infected duck brain. Totals of 117, 212, and 150 differentially expressed genes (DEGs) were identified at 12, 24, and 48 h post infection (hpi). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses uncovered genes and pathways related to the nervous system and immune responses in duck brain. Neuro-related genes, including WNT3A, GATA3, and CHRNA6, were found to be significantly downregulated. RIG-I-like receptors (DHX58, IFIH1) and Toll-like receptors (TLR2 and TLR3) were activated, inducing the expression of 22 interferon stimulated genes (ISGs) and antigen-processing and -presenting genes (TAP1 and TAP2) in the brain. Our research provides comprehensive information for the molecular mechanisms of neuro-immune and host-pathogen interactions of DTMUV.

摘要

鸭坦布苏病毒（DTMUV）是一种蚊媒黄病毒。它会导致鸭产蛋量严重下降，以及神经症状和脑损伤。然而，DTMUV 诱导神经毒力和宿主大脑反应的具体分子机制尚不清楚。为了更好地理解 DTMUV 感染的宿主-病原体和神经免疫相互作用，我们进行了高通量 RNA 测序，以揭示 DTMUV 感染鸭脑的转录组图谱。在感染后 12、24 和 48 小时，分别鉴定出 117、212 和 150 个差异表达基因（DEGs）。基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析揭示了与鸭脑中神经系统和免疫反应相关的基因和途径。神经相关基因，包括 WNT3A、GATA3 和 CHRNA6，被发现显著下调。RIG-I 样受体（DHX58、IFIH1）和 Toll 样受体（TLR2 和 TLR3）被激活，诱导大脑中 22 种干扰素刺激基因（ISGs）和抗原加工和呈递基因（TAP1 和 TAP2）的表达。我们的研究为 DTMUV 的神经免疫和宿主-病原体相互作用的分子机制提供了全面的信息。

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