Department of Biomedicine and Health Sciences, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, #222 Banpo-Daero Seocho-Gu, Seoul, 06591, Republic of Korea.
Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon, Korea.
MAGMA. 2020 Oct;33(5):735-746. doi: 10.1007/s10334-020-00840-w. Epub 2020 Apr 3.
The present study applied in vivo proton magnetic resonance spectroscopy (H MRS) to concurrently measure the concentration and T2 relaxation time of glutamate with the concept of optimized-for-quantification-and-T2-measurement-of-glutamate (OpQT2-Glu).
7T MRS scans of the OpQT2-Glu were acquired from the prefrontal cortex of five rats. The echo-time-(TE)-specific J-modulation of glutamate was investigated by spectral simulations and analyses for selecting the eight TEs appropriate for T2 estimation of glutamate. The OpQT2-Glu results were compared to those of the typical short-TE MRS and T2 measurements.
No significant differences were observed between the OpQT2-Glu and typical short-TE MRS (p > 0.050). The estimated glutamate T2 (67.75 ms) of the OpQT2-Glu was similar to the multiple TE MRS for the T2 measurement (71.58 ms) with enhanced signal-to-noise ratio and reliability.
The results revealed that the quantification reliability of the OpQT2-Glu was comparable to that of the single short-TE MRS and its estimation reliability for the T2 relaxation time of glutamate was enhanced compared to the multiple TE MRS for T2 measurement. Despite certain limitations, the quantification and T2 estimation of glutamate can be concurrently performed within an acceptable scan time via high-field in vivo H MRS with the OpQT2-Glu.
本研究应用活体质子磁共振波谱（1 H MRS），通过优化谷氨酸定量和 T2 测量（OpQT2-Glu）的概念，同时测量谷氨酸的浓度和 T2 弛豫时间。
对 5 只大鼠的前额叶皮质进行 OpQT2-Glu 的 7T MRS 扫描。通过光谱模拟和分析研究谷氨酸的回波时间（TE）特异性 J 调制，以选择适合谷氨酸 T2 估计的 8 个 TE。将 OpQT2-Glu 的结果与典型的短 TE MRS 和 T2 测量结果进行比较。
OpQT2-Glu 与典型短 TE MRS 之间无显著差异（p > 0.050）。OpQT2-Glu 估计的谷氨酸 T2（67.75 ms）与多 TE MRS 用于 T2 测量的结果（71.58 ms）相似，具有增强的信噪比和可靠性。
结果表明，OpQT2-Glu 的定量可靠性与单短 TE MRS 相当，与多 TE MRS 用于 T2 测量相比，其对谷氨酸 T2 弛豫时间的估计可靠性得到增强。尽管存在一定的局限性，但通过高场活体 1 H MRS 可以在可接受的扫描时间内同时进行谷氨酸的定量和 T2 估计。
