Division of Materials Science and Engineering, Boston University, Boston, MA, USA.
Department of Chemistry, Boston University, Boston, MA, USA.
Methods Mol Biol. 2020;2135:125-139. doi: 10.1007/978-1-0716-0463-2_7.
Small, stable, and bright quantum dots (QDs) are of interest in many biosensing and biomedical imaging applications, but current methodologies for obtaining these characteristics can be highly specialized or expensive. We describe a straightforward, low-cost protocol for functionalizing poly(isobutylene-alt-maleic anhydride) (PIMA) with moieties that anchor to the QD surface (histamine), impart hydrophilicity [(2-aminoethyl)trimethylammonium chloride (MeN-NH)], and provide a platform for biofunctionalization via click chemistry (dibenzocyclooctyne (DBCO)). Guidelines to successfully use this polymer for QD ligand exchange are presented, and an example of biofunctionalization with DNA is shown. Stable QD-DNA conjugates are obtained with high yield and without requiring additional purification steps.
小而稳定、发光性能良好的量子点 (QD) 在许多生物传感和生物医学成像应用中很受欢迎，但获得这些特性的当前方法可能非常专业或昂贵。我们描述了一种简单、低成本的方法，即用与 QD 表面结合的部分对聚异丁烯-马来酸酐 (PIMA) 进行功能化（组氨酸），赋予亲水性 [(2-氨基乙基)三甲基氯化铵 (MeN-NH)]，并通过点击化学（二苯并环辛炔 (DBCO)）提供生物功能化的平台。本文提出了成功使用该聚合物进行 QD 配体交换的指南，并展示了与 DNA 进行生物功能化的示例。通过该方法可以获得高产率且无需额外纯化步骤的稳定 QD-DNA 缀合物。
