CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience & Technology (NCNST), Beijing, 100190, PR China.
University of Chinese Academy of Sciences, Beijing, 100049, PR China.
Nanomedicine (Lond). 2020 Apr;15(10):1037-1061. doi: 10.2217/nnm-2019-0381. Epub 2020 Apr 6.
In spite of radical advances in nanobiotechnology, the clinical translation of nanoparticle (NP)-based agents is still a major challenge due to various physiological factors that influence their interactions with biological systems. Recent decade witnessed meticulous investigation on protein corona (PC) that is the first surrounds NPs once administered into the body. Formation of PC around NP surface exhibits resilient effects on their circulation, distribution, therapeutic activity, toxicity and other factors. Although enormous literature is available on the role of PC in altering pharmacokinetics and pharmacodynamics of NPs, understanding on its analytical characterization methods still remains shallow. Therefore, the current review summarizes the impact of PC on biological fate of NPs and stressing on analytical methods employed for studying the NP-PC.
尽管纳米生物技术取得了重大进展，但由于各种影响纳米粒子与生物系统相互作用的生理因素，基于纳米粒子的制剂的临床转化仍然是一个主要挑战。最近十年，人们对蛋白质冠（PC）进行了细致的研究，PC 是 NPs 进入体内后首先包裹的物质。PC 在 NP 表面的形成对其循环、分布、治疗活性、毒性和其他因素表现出有弹性的影响。尽管有大量文献研究了 PC 对 NPs 的药代动力学和药效动力学的改变作用，但对其分析表征方法的理解仍然很肤浅。因此，本综述总结了 PC 对 NPs 生物学命运的影响，并强调了用于研究 NP-PC 的分析方法。
