Ruhil Kamal, Prasad Sheo Mohan
1Sharda University, Plot No 32-34, Knowledge Park III, Greater Noida, Uttar Pradesh 201301 India.
2Lab No. 114, School of Life Sciences, Jawaharlal Nehru University, New Delhi, Delhi 110067 India.
Physiol Mol Biol Plants. 2020 Apr;26(4):841-856. doi: 10.1007/s12298-019-00754-5. Epub 2020 Feb 28.
Present study deals with responses of two cyanobacteria viz. and against butachlor [2-chloro-2,6-diethyl--(butoxymethyl) acetanilide] (low dose; 5 µg mL and high dose; 10 µg mL) and UV-B (7.2 kJ m) alone, and in combination. Butachlor and UV-B exposure, alone and in combination, suppressed growth of both the cyanobacteria. This was accompanied by inhibitory effect on whole cell oxygen evolution and photosynthetic electron transport activities. Both the stressors induced the oxidative stress as there was significant increase in superoxide radical (O ) and hydrogen peroxide (HO) contents resulting into increased lipid peroxidation and electrolyte leakage. In , low dose of butachlor and UV-B alone increased the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), while activity of all these enzymatic antioxidants declined significantly at treatments with high dose of butachlor alone, and with low and high doses of butachlor and UV-B in combination. In enhanced activity of SOD, CAT and POD (except POD at high dose of butachlor and UV-B combination) was noticed. Ascorbate level in declined progressively with increasing intensity of stress while in varied response was noticed. Proline contents increased progressively under tested stress in both the organisms. Overall results suggest that was more sensitive than against butachlor and UV-B stresses. Hence, may be preferred in paddy field for sustainable agriculture.
本研究探讨了两种蓝藻对丁草胺2-氯-2,6-二乙基-N-(丁氧甲基)乙酰苯胺以及单独的UV - B(7.2kJ/m²)和二者联合作用的反应。丁草胺和UV - B单独及联合作用均抑制了这两种蓝藻的生长。这伴随着对全细胞放氧和光合电子传递活性的抑制作用。两种应激源均诱导了氧化应激,因为超氧阴离子自由基(O₂⁻)和过氧化氢(H₂O₂)含量显著增加,导致脂质过氧化和电解质渗漏增加。在[蓝藻1名称]中,低剂量的丁草胺和单独的UV - B增加了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性,而在单独高剂量丁草胺处理以及低剂量和高剂量丁草胺与UV - B联合处理时,所有这些酶促抗氧化剂的活性均显著下降。在[蓝藻2名称]中,观察到SOD、CAT和POD活性增强(高剂量丁草胺和UV - B联合处理时POD除外)。[蓝藻1名称]中的抗坏血酸水平随着应激强度的增加而逐渐下降,而在[蓝藻2名称]中观察到不同的反应。在两种生物体中,脯氨酸含量在测试应激下均逐渐增加。总体结果表明,[蓝藻1名称]对丁草胺和UV - B胁迫比[蓝藻2名称]更敏感。因此,在稻田可持续农业中,[蓝藻2名称]可能更受青睐。