Ulrich Silvia, Grímsson Friđgeir
Department of Botany and Biodiversity Research, University of Vienna, Vienna, Austria.
Grana. 2019 Oct 24;59(1):44-57. doi: 10.1080/00173134.2019.1666915. eCollection 2020.
An advanced protocol to prepare single extant and fossil pollen grains for transmission electron microscopy (TEM) analysis allows for the fast recovery of data on the ultrastructure of pollen/spores. The protocol is easy to apply and less time consuming than previous methods. The 'loss' of pollen grains and pollen that is 'difficult to locate' within the embedding material is avoided, and each single pollen grain can be prepared successfully for TEM analysis. This preparation method is meant as an addition to the single-grain method using combined light and scanning electron microscopy to investigate dispersed fossil pollen grains developed by Dr Reinhard Zetter in the late 1980s.
一种用于透射电子显微镜（TEM）分析的制备单个现存和化石花粉粒的先进方案，能够快速获取有关花粉/孢子超微结构的数据。该方案易于应用，且比以前的方法耗时更少。避免了花粉粒的“损失”以及在包埋材料中“难以定位”的花粉，并且每个单个花粉粒都能成功制备用于TEM分析。这种制备方法是对20世纪80年代末由莱因哈德·泽特博士开发的使用光学显微镜和扫描电子显微镜相结合来研究分散化石花粉粒的单粒法的补充。
