Melchels Ferry P W, Dhert Wouter J A, Hutmacher Dietmar W, Malda Jos
Department of Orthopaedics, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands.
J Mater Chem B. 2014 Apr 28;2(16):2282-2289. doi: 10.1039/c3tb21280g. Epub 2014 Mar 17.
Additive manufacturing forms a potential route towards economically viable production of cellular constructs for tissue engineering. Hydrogels are a suitable class of materials for cell delivery and 3D culture, but are generally unsuitable as construction materials. Gelatine-methacrylamide is an example of such a hydrogel system widely used in the field of tissue engineering, e.g. for cartilage and cardiovascular applications. Here we show that by the addition of gellan gum to gelatine-methacrylamide and tailoring salt concentrations, rheological properties such as pseudo-plasticity and yield stress can be optimised towards gel dispensing for additive manufacturing processes. In the hydrogel formulation, salt is partly substituted by mannose to obtain isotonicity and prevent a reduction in cell viability. With this, the potential of this new bioink for additive tissue manufacturing purposes is demonstrated.
增材制造为组织工程中细胞构建体的经济可行生产提供了一条潜在途径。水凝胶是一类适用于细胞递送和3D培养的材料,但通常不适合作为建筑材料。明胶-甲基丙烯酰胺就是这样一种水凝胶系统的例子,它在组织工程领域广泛应用,例如用于软骨和心血管应用。在这里,我们表明,通过向明胶-甲基丙烯酰胺中添加结冷胶并调整盐浓度,可以优化诸如假塑性和屈服应力等流变特性,以实现用于增材制造工艺的凝胶分配。在水凝胶配方中,盐部分被甘露糖取代以获得等渗性并防止细胞活力降低。由此,证明了这种新型生物墨水用于增材组织制造目的的潜力。